Document Detail


The effect of a synthetic 7-thiaprostaglandin E1 derivative, TEI-6122, on monocyte chemoattractant protein-1 induced chemotaxis in THP-1 cells.
MedLine Citation:
PMID:  8564263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1 The ability of various prostaglandins (PGs) to inhibit monocyte chemotaxis induced by monocyte chemoattractant protein-1 (MCP-1) was investigated with a human monocytic leukaemia cell line, THP-1. Moreover, to investigate the mechanism of the inhibitory action of PGs the involvement of either intracellular adenosine 3': 5'-cyclic monosphosphate (cyclic AMP) accumulation or intracellular Ca2+ mobilization was studied. 2 TEI-6122, a synthetic 7-thia-PGE1 derivative, inhibited chemotaxis of THP-1 cells induced by MCP-1 with an IC50 of 1.5 pM. Its inhibitory activity was 1000 fold more than that of PGE1 and PGE2 (IC50 = 2.8 nM and 0.9 nM, respectively), which were more potent than other PGs such as PGA1, PGA2, PGF2 alpha and PGI2 (IC50 > or = 1 microM). 3 With respect to the effect on intracellular cyclic AMP accumulation in THP-1 cells, TEI-6122 was as potent as PGE1 and PGE2, which were approximately 100 to 1000 fold more potent than the other PGs such as PGA1, PGA2 and PGI2. The minimum concentration of TEI-6122 required to increase intracellular cyclic AMP accumulation in THP-1 cells was 1 nM. 4 TEI-6122 and PGE1 (4 microM) transiently increased intracellular calcium levels in THP-1 cells. When added prior to MCP-1, both PGs partially suppressed the increased in Ca2+ caused by this cytokine. There were no significant differences between the activity of TEI-6122 and PGE1 in either respect. 5 It is concluded that TEI-6122, a synthetic 7-thia-PGE1 derivative is a much more potent inhibitor of MCP-1-induced THP-1 cell chemotaxis than PGEI and PGE2 which are the best inhibitors among the natural PGs tested, while neither intracellular cyclic AMP accumulation nor effects on Ca2+ mobilization account for the extremely potent inhibitory activity of TEI-6122. Thus, either a novel PGE2 receptor (EPreceptor) or a novel intracellular signal transduction system may be involved in the extremely potent chemotaxis inhibitory activity of TEI-6122.
Authors:
H Tanaka; T Minoshima; N Endo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  116     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-03-05     Completed Date:  1996-03-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2298-302     Citation Subset:  IM    
Affiliation:
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alprostadil / analogs & derivatives*,  pharmacology
Calcium / metabolism
Chemokine CCL2 / antagonists & inhibitors*,  pharmacology
Chemotaxis, Leukocyte / drug effects*
Cyclic AMP / metabolism
Fibrinolytic Agents / pharmacology*
Humans
Kinetics
Leukemia, Monocytic, Acute / metabolism,  physiopathology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Fibrinolytic Agents; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; 745-65-3/Alprostadil; 83058-69-9/TFC 612
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