| The effect of surface modification of amorphous silica particles on NLRP3 inflammasome mediated IL-1beta production, ROS production and endosomal rupture. | |
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MedLine Citation:
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PMID: 20561679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although amorphous silica particles (SPs) are widely used in cosmetics, foods and medicinal products, it has gradually become evident that SPs can induce substantial inflammation accompanied by interleukin-1beta (IL-1beta) production. Here, to develop safe forms of SPs, we examined the mechanisms of SP-induced inflammation and the relationship between particle characteristics and biological responses. We compared IL-1beta production levels in THP-1 human macrophage like cells in response to unmodified SP of various diameters (30- to 1000-nm) and demonstrated that unmodified microsized 1000-nm SP (mSP1000) induced higher levels of IL-1beta production than did smaller unmodified SPs. Furthermore, we found that unmodified mSP1000-induced IL-1beta production was depended on the sequence of reactive oxygen species (ROS) production, endosomal rupture, and subsequent activation of pro-inflammatory complex NLRP3 inflammasome. In addition, we compared IL-1beta production levels in THP-1 cells treated with mSP1000s modified with a functional group (-COOH, -NH(2), -SO(3)H, -CHO). Although unmodified and surface-modified mSP1000s were taken up with similar frequencies equally into the THP-1 cells, surface modification of mSP1000 dramatically suppressed IL-1beta production by reducing ROS production. Our results reveal a part of NLRP3 activation pathway and provide basic information that should help to create safe and effective forms of SPs. |
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Authors:
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Tomohiro Morishige; Yasuo Yoshioka; Hiroshi Inakura; Aya Tanabe; Xinglei Yao; Shogo Narimatsu; Youko Monobe; Takayoshi Imazawa; Shin-ichi Tsunoda; Yasuo Tsutsumi; Yohei Mukai; Naoki Okada; Shinsaku Nakagawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-18 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 6833-42 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / metabolism* Caspase 1 / metabolism Cathepsin B / metabolism Cell Death / drug effects Cell Line Endosomes / drug effects, enzymology, pathology* Enzyme Activation / drug effects Female Humans Inflammation / metabolism*, pathology Interleukin-1beta / biosynthesis* Mice Mice, Inbred C57BL Monocytes / drug effects, enzymology, pathology, ultrastructure Particle Size Phagocytosis / drug effects Reactive Oxygen Species / metabolism* Silicon Dioxide / chemistry*, pharmacology* Surface Properties / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Interleukin-1beta; 0/NLRP3 protein, human; 0/Reactive Oxygen Species; 7631-86-9/Silicon Dioxide; EC 3.4.22.1/Cathepsin B; EC 3.4.22.36/Caspase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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