Document Detail


The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine.
MedLine Citation:
PMID:  20226519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
If skin's non-linear viscoelastic properties are mechanically exploited for precise antigen placement, there is tremendous promise for improved vaccines. To achieve this, we designed a Nanopatch-densely packed micro-nanoprojections (>20,000/cm(2)) to directly deposit antigen to large numbers of epidermal Langerhans cells and dermal dendritic cells. Here, we controllably applied our Nanopatches with discrete conditions between peak strain rates of approximately 100 s(-1)-7000 s(-1) and quantified resulting penetration depths, delivery payloads and skin mechanics. Increasing the strain rate of application, we overcame key skin variability, achieving increases in both projection penetration depth (by over 50% length) and area coverage of a full array (from 50% to 100%). This delivery depth precision opens the way for more fully utilizing the skin's immune function. Furthermore, we yielded new insights on mechanical behaviour of skin, including: 1) internal skin property changes that could affect/facilitate penetration; 2) projection design to dictate penetration depth; 3) puncture mechanics of skin in this strain rate range. Indeed, we show delivery of a model vaccine using our tested range of strain rates achieved functionally relevant tunable systemic antibody generation in mice. These findings could be of great utility in extending skin strata vaccine targeting approaches to human use.
Authors:
Michael L Crichton; Alexander Ansaldo; Xianfeng Chen; Tarl W Prow; Germain J P Fernando; Mark A F Kendall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-11
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  4562-72     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
The University of Queensland, Delivery of Drugs and Genes Group (D2G2), Australian Institute for Bioengineering and Nanotechnology, Brisbane, QLD 4072, Australia.
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MeSH Terms
Descriptor/Qualifier:
Administration, Cutaneous*
Animals
Coated Materials, Biocompatible / chemistry,  metabolism*
Drug Delivery Systems* / instrumentation,  methods
Female
Humans
Materials Testing
Mice
Mice, Inbred C57BL
Skin* / cytology,  immunology
Stress, Mechanical
Surface Properties
Vaccines / administration & dosage*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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