| The effect of storage on whole blood chemiluminescence measurement of equine neutrophils. | |
| | |
MedLine Citation:
|
PMID: 22730351 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to determine the effect of duration and temperature of sample storage on whole blood chemiluminescence measurement results. Venous blood from 18 clinically healthy Polish half-bred horses aged 4 to 11 years were used in the study. Luminol dependent chemiluminescence (CL) was used to measure neutrophil oxygen metabolism in whole blood. Blood samples were examined for spontaneous CL and stimulated by a surface receptor stimulus as well as extra-receptor stimulus. The assay was performed in two parallel experimental sets with samples stored at 4 and 22 °C, respectively. Whole blood CL was estimated at 2, 6, 24, 48, 72, 96 and 120 h after collection. The study demonstrated that temperature and duration of sample storage are factors that determine the quality of CL measurements of whole blood in horses. The study concluded that samples should be stored at 4 °C and the assay should be performed as early as possible. It was also shown that the viability period of horse blood for CL assays is relatively long. Material stored at room temperature for 24 h and even up to 48 h at 4 °C did not show any significant decrease in spontaneous or stimulated chemiluminescence. Copyright © 2012 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Wiesław Krumrych; Radosław Skórzewski; Edward Malinowski |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-6-22 |
Journal Detail:
|
Title: Luminescence : the journal of biological and chemical luminescence Volume: - ISSN: 1522-7243 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
|
Created Date: 2012-6-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100889025 Medline TA: Luminescence Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
|
Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, Bydgoszcz, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A survey on air bubble detector placement in the CPB circuit: a 2011 cross-sectional analysis of the...
Next Document: Effects of Cationic Structure on Cellulose Dissolution in Ionic Liquids: A Molecular Dynamics Study.