| The effect of storage of whole blood on the association of factor VIII-related antigen and factor VIII-coagulant antigen. | |
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MedLine Citation:
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PMID: 6818685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate the extent of denaturation of factor VIII-coagulant activity (VIII: C) during production of factor VIII concentrates, the factor VIII-coagulant antigen (VIII: CAg)/VIII: C ratio was measured in plasma, cryoprecipitate and cryosupernatant from fresh and stored blood. This ratio was close to unity for both cryoprecipitate and other concentrates, suggesting that VIII: CAg is lost concurrently with VIII: C during cryoprecipitation and further fractionation. Storage of blood (18 h, 22 degrees C) before processing resulted in a 30% loss of VIII: C from the separated plasma; however, VIII: CAg was not affected. In cryoprecipitate prepared from this plasma, VIII: C and VIII: CAg both were 30% lower than when prepared from fresh plasma. In the corresponding cryosupernatant, however, more VIII: CAg but less VIII: C was present compared with fresh material. Gel chromatography revealed that the rise of VIII: CAg in cryosupernatant prepared from stored blood, was due to an increased amount of VIII: CAg of low molecular weight, not being associated with factor VIII-related antigen. Such an increase in dissociated VIII: CAg was not detected in the plasma prior to cryoprecipitation. It is concluded that during storage of blood, molecular changes are induced in the factor VIII-VWF complex, possibly by limited proteolysis, which make the complex more liable to dissociation during subsequent cryoprecipitation. |
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Authors:
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J A Hellings; J Over; J A van Mourik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of haematology Volume: 29 ISSN: 0036-553X ISO Abbreviation: Scand J Haematol Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-03-11 Completed Date: 1983-03-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0404507 Medline TA: Scand J Haematol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 353-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens
/
analysis*,
isolation & purification,
standards Blood Preservation* Chemical Precipitation Chromatography, Gel Cryoglobulins / isolation & purification Factor VIII / analysis, immunology*, isolation & purification, standards Humans Plasma / analysis von Willebrand Factor |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Cryoglobulins; 0/von Willebrand Factor; 9001-27-8/Factor VIII |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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