Document Detail


The effect of storage on rat tissues and human plasma amino acid levels determined by HPLC.
MedLine Citation:
PMID:  3507236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue concentrations of the amino acids tryptophan, methionine, valine, phenylalanine, isoleucine and leucine were determined in rat liver, skeletal muscle and brain tissue and human plasma. The effect of storage at -20 degrees C for up to 21 days was examined. Significantly elevated levels after 7 and 21 days of storage were observed in liver and brain tissue but no changes were found in muscle and plasma. Minimal changes occurred in liver tissue stored up to 6 hours at -4 degrees C. This study emphasizes the need for rapid deproteinization of tissues to avoid changes in the free pool of amino acids.
Authors:
T Bottiglieri
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  2     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  1987  
Date Detail:
Created Date:  1989-05-03     Completed Date:  1989-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  195-6     Citation Subset:  IM    
Affiliation:
Department of Neurology, Institute of Psychiatry, De Crespigny Park, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids, Essential / blood*
Animals
Brain Chemistry
Chromatography, High Pressure Liquid
Freezing
Humans
Liver / analysis
Muscles / analysis
Preservation, Biological*
Rats
Rats, Inbred Strains
Specimen Handling
Time Factors
Chemical
Reg. No./Substance:
0/Amino Acids, Essential

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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