| The effect of storage on rat tissues and human plasma amino acid levels determined by HPLC. | |
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MedLine Citation:
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PMID: 3507236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tissue concentrations of the amino acids tryptophan, methionine, valine, phenylalanine, isoleucine and leucine were determined in rat liver, skeletal muscle and brain tissue and human plasma. The effect of storage at -20 degrees C for up to 21 days was examined. Significantly elevated levels after 7 and 21 days of storage were observed in liver and brain tissue but no changes were found in muscle and plasma. Minimal changes occurred in liver tissue stored up to 6 hours at -4 degrees C. This study emphasizes the need for rapid deproteinization of tissues to avoid changes in the free pool of amino acids. |
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Authors:
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T Bottiglieri |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 2 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 1987 |
Date Detail:
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Created Date: 1989-05-03 Completed Date: 1989-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 195-6 Citation Subset: IM |
Affiliation:
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Department of Neurology, Institute of Psychiatry, De Crespigny Park, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids, Essential
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blood* Animals Brain Chemistry Chromatography, High Pressure Liquid Freezing Humans Liver / analysis Muscles / analysis Preservation, Biological* Rats Rats, Inbred Strains Specimen Handling Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Essential |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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