| The effect of storage on allergen and microbial agent levels in frozen house dust. | |
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MedLine Citation:
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PMID: 12622747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: House dust samples collected for exposure studies are often stored for variable time periods until analysis. However, there is currently no information on the effects of dust storage on the content of biocontaminants. Therefore, associations were analysed between the levels of mite allergens (Der p 1, Der f 1), cat allergen (Fel d 1) and microbial components (endotoxin, beta(1-->3)-glucan) on the one hand and the storage duration of dust samples at -20 degrees C on the other hand. METHODS: Within the framework of a study on the influences of INdoor factors and Genetics on Asthma (INGA), dust samples were collected from living room floors between June 1995 and August 1998 and extracted according to a standardized protocol. The concentrations of Der p 1, Der f 1, Fel d 1 and beta(1-->3)-glucan were determined with specific enzyme immunoassays. Endotoxin content was quantified using a chromogenic kinetic Limulus amoebocyte lysate (LAL) test. All concentrations were expressed per gram of dust RESULTS: Dust samples (n = 1236) were obtained from 655 homes in Hamburg, Hettstedt, Zerbst and Bitterfeld. Storage duration (range 8-298 days) was grouped into four categories (<or= 60 d; 61-90 d; 91-120 d; > 120 d). After adjustment for city of residence and season of dust sampling, means ratios comparing categories 2-4 to the first category were not statistically significant for Der p 1, Der f 1, endotoxin and beta(1-->3 glucan). However, Fel d 1 concentrations significantly declined with increased storage times of dust samples. CONCLUSIONS: Storage of house dust at -20 degrees C for up to 10 months has no effect on mite allergen, endotoxin and beta(1-->3)-glucan levels. A potential loss of Fel d 1 during storage of frozen dust samples needs further investigations by repeated measurements of allergen in identical dust samples. |
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Authors:
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B Fahlbusch; A Koch; J Douwes; W Bischof; U Gehring; K Richter; H-E Wichmann; J Heinrich |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Allergy Volume: 58 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-03-07 Completed Date: 2003-07-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
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Languages: eng Pagination: 150-3 Citation Subset: IM |
Affiliation:
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Friedrich-Schiller-University of Jena, Institute of Immunology, Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Air Pollution, Indoor
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analysis Allergens / analysis*, immunology* Analysis of Variance Animals Antigens, Dermatophagoides / analysis, immunology Asthma / immunology Dose-Response Relationship, Immunologic Endotoxins / analysis, immunology Germany Glucans / analysis, immunology Glycoproteins / analysis, immunology Humans Pyroglyphidae / immunology* Statistics as Topic Time Factors beta-Glucans* |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Antigens, Dermatophagoides; 0/Endotoxins; 0/Fel d 1 protein, Felis domesticus; 0/Glucans; 0/Glycoproteins; 0/beta-Glucans; 9051-97-2/beta-1,3-glucan; EC 3.4.22.-/Dermatophagoides farinae antigen f 1; EC 3.4.22.-/Dermatophagoides pteronyssinus antigen p 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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