Document Detail


The effect of stimuli on basophil-mediated atopic responses during asthmatic lying-in women and in newborns.
MedLine Citation:
PMID:  22894778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morbidity from allergic diseases is increasing. Basophils play a critical role in systemic anaphylaxis and chronic allergic inflammation. The prenatal environment must be regarded as a possible early risk factor for allergic diseases in children. Our objective was to determine if basophils harvested from neonates genetically predisposed to atopic disease had different levels of CD63 expression and IL-4 release properties in response to various stimuli (peptidoglycan, Dermatophagoides farinae, hyperosmotic mannitol). Blood samples were collected from 16 asthmatic and 18 healthy women and their newborns. Peripheral blood basophil histamine was measured using the human basophil degranulation test (HBDT), whereas activation was assessed by flow cytometric measurement of CD63 expression on the cord blood basophil surface. IL-4 levels were quantified by ELISA following allergen stimulation. The basophil degranulation index (DI) in granulocytes harvested from the peripheral blood of asthmatic women was assessed following stimulation with peptidoglycan (PGN), Dermatophagoides farinae (Df ) extract, or hyperosmotic mannitol. The DI was significantly higher in atopic women than in healthy controls. Upregulation of CD63 on the cord blood basophil surface was also detected in response to these stimuli. Basophils purified from the cord blood of neonates born to atopic mothers produced more IL-4 compared to basophils purified from the controls. These data suggested that various stimuli play a role in augmenting allergic reactions via modulation of activated basophil cytokine secretion. It may require new methods to stabilize the basophils in allergic diseases.
Authors:
Ling Yang; Yin-Shi Guo; Jin-Qi Jiang; Xue-Jun Guo; Yi-Ping Xu; Ye Tian; Ying Xiong; Li Han
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hybridoma (2005)     Volume:  31     ISSN:  1557-8348     ISO Abbreviation:  Hybridoma (Larchmt)     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-16     Completed Date:  2012-12-26     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  101241539     Medline TA:  Hybridoma (Larchmt)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
Affiliation:
Geriatrics Department, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD63 / metabolism
Antigens, Dermatophagoides / immunology
Asthma / immunology*,  metabolism,  pathology
Basophil Degranulation Test
Basophils / immunology*,  metabolism,  physiology
Case-Control Studies
Cells, Cultured
Female
Fetal Blood / cytology
Histamine Release
Humans
Infant, Newborn
Interleukin-4 / metabolism,  secretion
Mannitol / immunology
Peptidoglycan / immunology
Chemical
Reg. No./Substance:
0/Antigens, CD63; 0/Antigens, Dermatophagoides; 0/CD63 protein, human; 0/IL4 protein, human; 0/Peptidoglycan; 207137-56-2/Interleukin-4; 69-65-8/Mannitol
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