Document Detail

The effect of stimulators and blockers of adrenergic receptors on LH secretion and cyclic nucleotide (cAMP and cGMP) production by porcine pituitary cells in vitro.
MedLine Citation:
PMID:  11755719     Owner:  NLM     Status:  MEDLINE    
The direct effects of alpha- and beta-adrenergic agents on luteinizing hormone (LH) secretion in vitro by porcine pituitary cells and the participation of secondary messengers, adenosine 3'5'-monophosphate (cAMP) and guanosine 3'5'-monophospate (cGMP), in transduction of signals induced by adrenergic agents and gonadotropin-releasing hormone (GnRH) in these cells have been investigated. Pituitary glands were obtained from mature gilts, which were ovariectomized (OVX) 1 month before slaughter. OVX gilts, assigned to four groups, were primed with: (1) vehicle (OVX); (2 and 3) estradiol benzoate (EB; 2.5mg/100kg b.w.) at 30-36h (OVX+EB I) or 60-66h (OVX+EB II) before slaughter, respectively; (4) progesterone (P(4); 120mg/100kg b.w.) for 5 consecutive days before slaughter (OVX+P(4)). Anterior pituitaries were dispersed with trypsin and then pituitary cells were cultured (10(6) per well) in McCoy's 5a medium containing horse serum (10%) and fetal calf serum (2.5%) for 3 days, at 37 degrees C and under the atmosphere of 95% air and 5% CO(2). On day 4 of the culture, the cells were submitted to 3.5h incubation in the presence of GnRH (a positive control), alpha- and beta-adrenergic agonists (phenylephrine (PHEN) and isoproterenol (ISOP), respectively), and alpha- and beta-adrenergic blockers (phentolamine (PHENT) and propranolol (PROP), respectively). The culture media were assayed for LH (experiment I) and cyclic nucleotides (experiment II). In experiment I, addition of GnRH (100ng/ml) increased LH secretion by pituitary cells taken from gilts of all experimental groups. The effects of alpha- and beta-adrenergic agents on LH secretion by the cells depended on hormonal status of gilts. The LH secretion by pituitary cells of OVX gilts was potentiated in the presence of PHEN (10, 100nM, and 1microM) and PHENT (1microM), alone or in combination with PHEN (100nM) and by the cells derived from OVX+EB I and OVX+P(4) animals in response to PHEN (100nM) and ISOP (1microM). ISOP (1microM) also stimulated LH secretion by the cells taken from OVX+EB II gilts. In experiment II, GnRH (100ng/ml) increased cGMP production by pituitary cells obtained from all groups of gilts and cAMP secretion by the cells taken from OVX and OVX+P(4) animals. PHEN (100nM) decreased and PROP (1microM) enhanced cAMP production by pituitary cells derived from OVX+EB I and OVX gilts, respectively. Moreover, PHEN (100nM) reduced, while PHENT (1microM) stimulated the release of cGMP by pituitary cells taken from OVX+EB II animals. In turn, ISOP (100nM) decreased and increased cGMP production by the cells derived from OVX+EB II and OVX+P(4) gilts, respectively. PROP (1microM) potentiated cGMP accumulation by pituitary cells taken from OVX+EB I and OVX+P(4) animals. In conclusion, our results suggest that adrenergic agents can modulate LH release by porcine pituitary cells acting through guanyl and adenylyl cyclase and in a manner dependent on hormonal status of gilts.
G Siawrys; I Bogacka; S Okrasa; T Kaminski; J Przala
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal reproduction science     Volume:  69     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-05-21     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-89     Citation Subset:  IM    
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Kortowo 5, 10-718, Olsztyn, Poland.
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MeSH Terms
Adrenergic Agonists / pharmacology
Adrenergic Antagonists / pharmacology*
Cells, Cultured
Cyclic AMP / physiology,  secretion*
Cyclic GMP / physiology,  secretion*
Gonadotropin-Releasing Hormone / pharmacology
Luteinizing Hormone / drug effects,  secretion*
Pituitary Gland / cytology,  drug effects,  secretion*
Random Allocation
Second Messenger Systems / physiology
Signal Transduction / physiology
Swine / physiology*
Reg. No./Substance:
0/Adrenergic Agonists; 0/Adrenergic Antagonists; 33515-09-2/Gonadotropin-Releasing Hormone; 60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP; 9002-67-9/Luteinizing Hormone

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