Document Detail

The effect of skeletal maturity on the regenerative function of intrinsic ACL cells.
MedLine Citation:
PMID:  19890988     Owner:  NLM     Status:  MEDLINE    
Anterior cruciate ligament (ACL) injuries are an important clinical problem, particularly for adolescent patients. The effect of skeletal maturity on the potential for ACL healing is as yet unknown. In this study, we hypothesized that fibroblastic cells from the ACLs of skeletally immature animals would proliferate and migrate more quickly than cells from adolescent and adult animals. ACL tissue from skeletally immature, adolescent, and adult pigs and sheep were obtained and cells obtained using explant culture. Cell proliferation within a collagen-platelet scaffold was measured at days 2, 7, and 14 of culture using AM MTT assay. Cellular migration was measured at 4 and 24 h using a modified Boyden chamber assay, and cell outgrowth from the explants also measured at 1 week. ACL cells from skeletally immature animals had higher proliferation between 7 and 14 days (p<0.01 for all comparisons) and higher migration potential at all time points in both species (p<0.01 for all comparisons). ACL cells from skeletally immature animals have greater cellular proliferation and migration potential than cells from adolescent or adult animals. These experiments suggest that skeletal maturity may influence the biologic repair capacity of intrinsic ACL cells.
Ashley N Mastrangelo; Elise M Magarian; Matthew P Palmer; Patrick Vavken; Martha M Murray
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  28     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-04-13     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  644-51     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Orthopaedic Research Society.
Department of Orthopaedic Surgery, Enders 1020, 300 Longwood Avenue, Children's Hospital, Boston, Boston, Machusetts 02115, USA.
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MeSH Terms
Age Factors
Anterior Cruciate Ligament* / cytology,  injuries,  physiology
Bone Development
Cell Division / physiology
Cell Movement / physiology
Cells, Cultured
Culture Media / pharmacology
Diffusion Chambers, Culture
Fibroblasts / cytology*,  physiology*
Platelet-Rich Plasma
Regeneration / physiology*
Species Specificity
Sus scrofa
Wound Healing / physiology*
Grant Support
K02 AR049346/AR/NIAMS NIH HHS; K02 AR049346-05/AR/NIAMS NIH HHS; R01 AR054099/AR/NIAMS NIH HHS; R01 AR054099-05/AR/NIAMS NIH HHS; R03 AR046356-01/AR/NIAMS NIH HHS
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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