Document Detail

The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
MedLine Citation:
PMID:  18095366     Owner:  NLM     Status:  MEDLINE    
The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to significantly change their expression, the great majority of protein changes were related to the cytoskeleton, the stress response and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell culture can adapt themselves to environmental changes, without significantly altering cell viability, at least after a few days of treatment, even though serum withdrawal represents a stress condition capable to increase ROS production, to influence cell metabolism and to interfere with cell behaviour, favouring the expression of several age-related features.
Federica Boraldi; Giulia Annovi; Chiara Paolinelli-Devincenzi; Roberta Tiozzo; Daniela Quaglino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  8     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-26     Completed Date:  2008-03-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  66-82     Citation Subset:  IM    
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
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MeSH Terms
Amino Acid Sequence
Cell Culture Techniques*
Cells, Cultured
Fibroblasts / chemistry,  metabolism*
G0 Phase / physiology
Molecular Sequence Data
Proteins / metabolism*
Proteome / analysis*
Skin / chemistry,  cytology*,  metabolism
Reg. No./Substance:
0/Proteins; 0/Proteome

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