Document Detail


The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines.
MedLine Citation:
PMID:  16123787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb)cell lines. METHODS: Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by immunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. The averaged results per condition were recorded. The Student's t-test was used to compare results from the cells cultured with and without amfenac. RESULT: The Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. The two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immunocytochemical analysis. CONCLUSION: The selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.
Authors:
J P de Souza Filho; Z M S Correa; J C Marshall; E Anteka; A B Coutinho; M C Martins; M N Burnier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Eye (London, England)     Volume:  20     ISSN:  0950-222X     ISO Abbreviation:  Eye (Lond)     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-04     Completed Date:  2006-12-07     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8703986     Medline TA:  Eye (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  598-601     Citation Subset:  IM    
Affiliation:
The Henry C Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada. jpessoafilho@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Proliferation / drug effects
Cyclooxygenase 2 / metabolism
Cyclooxygenase 2 Inhibitors / pharmacology*
Humans
Phenylacetates / pharmacology*
Retinal Neoplasms / enzymology,  pathology*
Retinoblastoma / enzymology,  pathology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Cyclooxygenase 2 Inhibitors; 0/Phenylacetates; 51579-82-9/amfenac; EC 1.14.99.1/Cyclooxygenase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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