Document Detail

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells.
MedLine Citation:
PMID:  15588648     Owner:  NLM     Status:  MEDLINE    
In order to evaluate the seasonal variation as well as cytotoxicity of different fractions of Alstonia scholaris R. Br. (ASE), the HeLa cells were treated with different doses of various fractions of ASE collected in monsoon, winter and summer. The exposure of HeLa cells to different extracts prepared from the stem bark collected in monsoon, winter and summer seasons resulted in a dose dependent increase in the cell killing effect of ASE and the highest cell killing effect was observed for the extract prepared from the summer collections. Similarly, treatment of HeLa cells with different doses of various fractions of the Alstonia scholaris extract viz. residue (ASERS), steroidal (ASEST), chloroform (ASECH), petroleum ether (ASEPE), diethyl ether (ASEDE), ethyl acetate (ASEEA), n-butanol (ASENB), aqueous (ASEAQ) and echitamine chloride (ECL) also resulted in a dose dependent decline in the cell viability, where the greatest cytotoxic effect was observed for residue (ASERS), followed by the whole extract (ASE) and chloroform (ASECH) fraction, while the least activity was observed for the steroidal (ASEST) fraction. The cytotoxicity declined ASERS > ASE > ASECH >ECL > ASEEA > ASEDE > ASEPE > ASENB > ASEAQ > ASEST in order. Our study demonstrates that the extract prepared from the summer collection, and the fractions containing the alkaloids were highly effective in cell killing. The extract of ASE was more powerful than the active principle echitamine present in ASE.
Ganesh Chandra Jagetia; Manjeshwar Shrinath Baliga
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  96     ISSN:  0378-8741     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-06-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  37-42     Citation Subset:  IM    
Department of Radiobiology, Kasturba Medical College, Manipal 576 104, India.
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MeSH Terms
Antineoplastic Agents / chemistry,  pharmacology*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Hela Cells
Plant Bark / chemistry
Plant Extracts / administration & dosage,  chemistry,  pharmacology
Reg. No./Substance:
0/Antineoplastic Agents; 0/Plant Extracts

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