Document Detail


The effect of sample composition and vial type on Cerenkov counting in a liquid scintillation counter.
MedLine Citation:
PMID:  6660484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of sample vial type and sample composition on the Cerenkov count rate detected from 32P and 36Cl was studied using a liquid scintillation counter. When counting was done in the noncoincident mode, glass vials allowed higher counting efficiency than plastic vials. In the coincident mode light scattering caused by polyethylene and polyproplyene vials allowed higher counting efficiency than glass vials. Highest coincident counting efficiency was from plastic minivials in a glass carrier vial. Increased solute concentration in samples caused increased counting efficiency due to changes in the refractive index of the solution. This can cause significant counting efficiency changes with no sample channel ratio change in density gradient fractions. The use of wavelength shifters is shown to be inappropriate when the sample pH varies, as this can change the fluorescent properties of the shifters and thereby the observed count rate.
Authors:
T F Kellogg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  134     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chlorine
Hydrogen-Ion Concentration
Osmolar Concentration
Phosphorus Radioisotopes
Radioisotopes
Scintillation Counting / instrumentation,  methods*
Solutions
Chemical
Reg. No./Substance:
0/Phosphorus Radioisotopes; 0/Radioisotopes; 0/Solutions; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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