| The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation. | |
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MedLine Citation:
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PMID: 22809850 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading. |
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Authors:
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Enda P Dowling; William Ronan; Gidon Ofek; Vikram S Deshpande; Robert M McMeeking; Kyriacos A Athanasiou; J Patrick McGarry |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-18 |
Journal Detail:
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Title: Journal of the Royal Society, Interface / the Royal Society Volume: - ISSN: 1742-5662 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101217269 Medline TA: J R Soc Interface Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Mechanical and Biomedical Engineering, National University of Ireland, , University Road, Galway, Republic of Ireland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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