Document Detail


The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation.
MedLine Citation:
PMID:  22809850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading.
Authors:
Enda P Dowling; William Ronan; Gidon Ofek; Vikram S Deshpande; Robert M McMeeking; Kyriacos A Athanasiou; J Patrick McGarry
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-18
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  9     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-26     Completed Date:  2013-06-19     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  3469-79     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism*,  physiology
Animals
Biomechanical Phenomena
Cattle
Chondrocytes / cytology,  physiology*,  ultrastructure
Computer Simulation*
Models, Biological*
Stress Fibers / metabolism,  physiology
Stress, Mechanical
Comments/Corrections

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