| The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus. | |
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MedLine Citation:
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PMID: 22535652 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVES: Differential Normalized Fluorescence (DNF) technique has been used to distinguish high-grade dysplasia from non-dysplastic Barrett's esophagus. This technology may assist gastroenterologists in targeting biopsies, reducing the number of biopsies using the standard protocol. In the presence of reactive atypia/inflammation, it becomes difficult for the pathologist to differentiate non-dysplastic Barrett's esophagus from Barrett's esophagus with low-grade dysplasia. Before DNF technique may be used to guide target biopsies, it is critical to know whether reactive atypia/inflammation in non-dysplastic Barrett's may result in false positives. This study was conducted to determine whether DNF technique is adversely affected by the presence of reactive atypia/inflammation in non-dysplastic Barrett's esophagus resulting in false positives. STUDY DESIGN/MATERIALS AND METHODS: Four hundred ten-nanometer laser light was used to induce autofluorescence of Barrett's mucosa in 49 patients. The clinical study included 37 males and 12 females. This was a blinded retrospective data analysis study. A total of 303 spectra were collected and matched to non-dysplastic Barrett's biopsy results. One hundred seventy-five spectra were collected from areas with a pathology of non-dysplastic Barrett's esophagus with reactive atypia/inflammation. One hundred twenty-eight spectra were collected from areas with non-dysplastic Barrett's esophagus without reactive changes/inflammation. The spectra were analyzed using the DNF Index at 480 nm and classified as positive or negative using the threshold of -0.75 × 10(-3). RESULTS: Using DNF technique, 92.6% of non-dysplastic samples with reactive atypia/inflammation were classified correctly (162/175). 92.2% of non-dysplastic samples without reactive atypia/inflammation were classified correctly (118/128). Comparing the ratios of false positives among the two sample groups, there was not a statistically significant difference between the two groups. CONCLUSION: Using DNF technique for classification of non-dysplastic Barrett's mucosa does not result in false-positive readings due to reactive atypia/inflammation. Target biopsies guided by DNF technique may drastically reduce the number of pinch biopsies using the standard biopsy protocol. |
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Authors:
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Masoud Panjehpour; Bergein F Overholt; Tuan Vo-Dinh; Domenico Coppola |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2012-04-25 |
Journal Detail:
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Title: Lasers in surgery and medicine Volume: 44 ISSN: 1096-9101 ISO Abbreviation: Lasers Surg Med Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-08 Completed Date: 2012-10-11 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8007168 Medline TA: Lasers Surg Med Country: United States |
Other Details:
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Languages: eng Pagination: 390-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Laser Center, Thompson Cancer Survival Center, Knoxville, TN 37916, USA. mpanjehp@covhlth.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Barrett Esophagus
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complications,
pathology* Biopsy Diagnosis, Differential Esophagoscopy / instrumentation, methods* Esophagus / pathology* False Positive Reactions Female Humans Inflammation / etiology Lasers, Dye / diagnostic use* Male Retrospective Studies Single-Blind Method Spectrometry, Fluorescence / instrumentation, methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA088787/CA/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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