Document Detail


The effect of raft lipid depletion on microvilli formation in MDCK cells, visualized by atomic force microscopy.
MedLine Citation:
PMID:  15135052     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated whether raft lipids of Madin-Darby canine kidney (MDCK) cells play any role in microvilli maintenance using a combination of atomic force microscopy (AFM) and laser scanning confocal microscopy. MDCK cells were treated to reduce the amount of sphingolipids, cholesterol, or both and subsequently imaged, in buffer solution, using AFM. It was observed that inhibition of either sphingolipid or cholesterol biosynthesis led to a reduction in the number of microvilli on the surface of MDCK cells. However, this effect was not uniform across the monolayer, with some cells resembling those in untreated controls. The subsequent extraction of cholesterol from cells grown in the presence of inhibitors led to a further reduction in microvilli on the surface of the cells and, in some cases, resulted in monolayers devoid of full length microvilli. Significantly, smaller spikes were observed on the surface of the smoother cells.
Authors:
Kate Poole; Doris Meder; Kai Simons; Daniel Müller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  565     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2004-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Affiliation:
Max Planck Institute for Molecular and Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany. poole@mpi-cbg.de
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MeSH Terms
Descriptor/Qualifier:
Actins / chemistry
Animals
Cell Line
Cell Membrane / ultrastructure
Cholesterol / chemistry
Dogs
Image Processing, Computer-Assisted
Lasers
Membrane Microdomains / chemistry*
Microscopy, Atomic Force / methods*
Microscopy, Confocal / methods*
Microvilli / ultrastructure*
Sphingolipids / chemistry
Chemical
Reg. No./Substance:
0/Actins; 0/Sphingolipids; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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