Document Detail


The effect of protein kinase C-delta knockdown on anti-free radical enzyme and neuropeptide Y gene expression in phenylpropanolamine-treated rats.
MedLine Citation:
PMID:  20533995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypothalamic neuropeptide Y (NPY) has been reported to involve in regulating behavioral response of phenylpropanolamine (PPA), a sympathomimetic agent. This study explored if protein kinase C (PKC)-delta signaling participated in this regulation. Moreover, possible roles of anti-free radical enzyme catalase (CAT) and nitrogen oxide synthase (NOS) were also examined. Rats were treated daily with PPA for 4 days. Changes in food intake and hypothalamic NPY, PKCdelta, CAT, and NOS contents were assessed and compared. Results showed that PKCdelta and CAT increased during PPA treatment, which were concomitant with decreases in NPY content and food intake, while the change of NOS was expressed differently. Moreover, PKCdelta knockdown could modify PPA anorexia as well as NPY and CAT expression, while NOS expression remained unchanged. Furthermore, pre-treatment with NOS inhibitor could modify both PPA anorexia and NPY content. It is suggested that PKCdelta participates in the anorectic response of PPA via the modulation of NPY and CAT, while NOS contribute to this modulation via a different mechanism during PPA treatment. Results provide molecular mechanism of NPY-mediated PPA anorexia and may aid the therapeutic research of PPA and other anti-obesity drugs.
Authors:
Dong-Yih Kuo; Shun-Fa Yang; Shu-Chen Chu; Chin-Hsiu Chen; Pei-Ni Chen; Yih-Shou Hsieh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-01
Journal Detail:
Title:  Journal of neurochemistry     Volume:  114     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1217-30     Citation Subset:  IM    
Affiliation:
Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City, Taiwan, China. dykuo@csmu.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Anorexia / chemically induced,  enzymology,  genetics
Catalase / metabolism*
Disease Models, Animal
Eating / drug effects
Free Radicals / antagonists & inhibitors
Gene Expression Regulation / drug effects,  physiology
Hypothalamus / drug effects*,  metabolism,  physiopathology
Male
Neuropeptide Y / biosynthesis,  genetics*,  physiology
Nitric Oxide / antagonists & inhibitors,  biosynthesis
Nitric Oxide Synthase Type I / antagonists & inhibitors,  physiology*
Oxidative Stress / drug effects,  physiology
Phenylpropanolamine / administration & dosage*
Protein Kinase C-delta / deficiency*,  genetics*,  physiology
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Neuropeptide Y; 10102-43-9/Nitric Oxide; 14838-15-4/Phenylpropanolamine; EC 1.11.1.6/Catalase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 2.7.11.13/Protein Kinase C-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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