| The effect of protein kinase C-delta knockdown on anti-free radical enzyme and neuropeptide Y gene expression in phenylpropanolamine-treated rats. | |
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MedLine Citation:
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PMID: 20533995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypothalamic neuropeptide Y (NPY) has been reported to involve in regulating behavioral response of phenylpropanolamine (PPA), a sympathomimetic agent. This study explored if protein kinase C (PKC)-delta signaling participated in this regulation. Moreover, possible roles of anti-free radical enzyme catalase (CAT) and nitrogen oxide synthase (NOS) were also examined. Rats were treated daily with PPA for 4 days. Changes in food intake and hypothalamic NPY, PKCdelta, CAT, and NOS contents were assessed and compared. Results showed that PKCdelta and CAT increased during PPA treatment, which were concomitant with decreases in NPY content and food intake, while the change of NOS was expressed differently. Moreover, PKCdelta knockdown could modify PPA anorexia as well as NPY and CAT expression, while NOS expression remained unchanged. Furthermore, pre-treatment with NOS inhibitor could modify both PPA anorexia and NPY content. It is suggested that PKCdelta participates in the anorectic response of PPA via the modulation of NPY and CAT, while NOS contribute to this modulation via a different mechanism during PPA treatment. Results provide molecular mechanism of NPY-mediated PPA anorexia and may aid the therapeutic research of PPA and other anti-obesity drugs. |
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Authors:
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Dong-Yih Kuo; Shun-Fa Yang; Shu-Chen Chu; Chin-Hsiu Chen; Pei-Ni Chen; Yih-Shou Hsieh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-01 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 114 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1217-30 Citation Subset: IM |
Affiliation:
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Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City, Taiwan, China. dykuo@csmu.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anorexia / chemically induced, enzymology, genetics Catalase / metabolism* Disease Models, Animal Eating / drug effects Free Radicals / antagonists & inhibitors Gene Expression Regulation / drug effects, physiology Hypothalamus / drug effects*, metabolism, physiopathology Male Neuropeptide Y / biosynthesis, genetics*, physiology Nitric Oxide / antagonists & inhibitors, biosynthesis Nitric Oxide Synthase Type I / antagonists & inhibitors, physiology* Oxidative Stress / drug effects, physiology Phenylpropanolamine / administration & dosage* Protein Kinase C-delta / deficiency*, genetics*, physiology Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Neuropeptide Y; 10102-43-9/Nitric Oxide; 14838-15-4/Phenylpropanolamine; EC 1.11.1.6/Catalase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 2.7.11.13/Protein Kinase C-delta |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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