| The effect of prolactin on murine in vitro fertilization and embryo development. | |
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MedLine Citation:
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PMID: 2360588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elevated levels of serum and follicular fluid prolactin occur in women undergoing ovulation induction with both clomiphene citrate and gonadotropin therapy. Prolactin's effect on oocyte fertilization and embryo cleavage has not been fully characterized. Using a murine model, we investigated the effect of prolactin on in vitro fertilization and subsequent embryo cleavage in media containing 150, 400, and 600 ng/ml purified mouse prolactin. No difference was found in fertilization rates when compared with control rates. Culture of both in vivo and in vitro fertilized two-cell embryos in murine prolactin at 150, 400, and 600 ng/ml showed no significant difference in blastocyst, morula, or embryo degeneration rates when compared with control rates. An assay for binding of murine prolactin to spermatozoa, oocytes, and the embryo at various cleavage stages revealed no specific murine prolactin binding. These in vitro experimental results fail to show a role for murine prolactin in effecting mature oocyte fertilization or subsequent embryo cleavage. The lack of binding of murine prolactin to the gametes and early developing embryo supports the in vitro findings. |
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Authors:
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W G Dodds; J Fowler; A Peykoff; K F Miller; C I Friedman; M H Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 162 ISSN: 0002-9378 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-08-02 Completed Date: 1990-08-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1553-9; discussion 1559-61 Citation Subset: AIM; IM |
Affiliation:
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Department of Obstetrics and Gynecology, Ohio State University Hospitals, Columbus, OH 43210. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Embryo, Mammalian / drug effects* Embryonic and Fetal Development Female Fertilization / drug effects Fertilization in Vitro / drug effects* Male Mice Mice, Inbred Strains Models, Biological Oocytes / drug effects Prolactin / blood, pharmacology* Spermatozoa / drug effects |
| Chemical | |
Reg. No./Substance:
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9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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