Document Detail

The effect of prolactin on estradiol-17 beta and testosterone plus 5 alpha-dihydrotestosterone secretion by porcine luteal cells in vitro.
MedLine Citation:
PMID:  6540702     Owner:  NLM     Status:  MEDLINE    
Prolactin (1, 5, 10 micrograms/ml) inhibited 5 alpha-dihydrotestosterone (DHT), testosterone (T) and estradiol-17 beta (E2) secretion by luteal cells isolated from porcine corpus luteum on the 13th day of the estrous cycle. The effect of prolactin upon luteal cells on the 5th and 17th day of the cycle was different. Inhibition of DHT, T and E2 secretion in luteal cells on the 5th day of the cycle occurred only after 6 hours of incubation, at prolactin doses of 5 and 10 micrograms/ml of the medium. Luteal cells obtained from corpora lutea with considerable physiological luteolysis (17th day of the cycle) did not react to the presence of prolactin in the incubation medium. The results showed that the effect of prolactin upon luteal cells depended on the intensity of steroidogenesis. Luteal cells on the 5th day of the estrous cycle produced progesterone in the amounts 34 +/- 9.1 ng/ml, on the 13th day: 62 +/- 11.5, and on the 17th day: 7.9 +/- 1.9. The effect of prolactin was most pronounced in case of cells on the 13th day of the cycle. These cells were characterized by the highest production of progesterone. The in vitro observations suggest the role of prolactin in inhibiting synthesis or release of DHT, T and E2 from luteal cells.
J Przała; T Wiesak; A Grazul; E Cieplińska
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental and clinical endocrinology     Volume:  83     ISSN:  0232-7384     ISO Abbreviation:  Exp. Clin. Endocrinol.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-10-10     Completed Date:  1984-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302802     Medline TA:  Exp Clin Endocrinol     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  343-8     Citation Subset:  IM    
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MeSH Terms
Corpus Luteum / drug effects*,  secretion
Dihydrotestosterone / secretion*
Estradiol / secretion*
Prolactin / pharmacology*
Testosterone / secretion*
Reg. No./Substance:
50-28-2/Estradiol; 521-18-6/Dihydrotestosterone; 58-22-0/Testosterone; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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