Document Detail


The effect of processing and cryopreservation on nucleated umbilical cord blood cells.
MedLine Citation:
PMID:  15493721     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque or Puregene red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 +/- 10% (average +/- standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 +/- 20% compared to 91+/-11% (p<0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen.
Authors:
Lucy A Bayer-Zwirello; Despina E Hoffman; Lorrie A Adams; Paul T Wilder; Margaret T Reece
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of perinatal medicine     Volume:  32     ISSN:  0300-5577     ISO Abbreviation:  J Perinat Med     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-20     Completed Date:  2005-01-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0361031     Medline TA:  J Perinat Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  430-3     Citation Subset:  IM    
Affiliation:
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth's Medical Center, Boston, MA 02135, U.S.A. zwirello@massmed.org
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD34
Antigens, CD45
Blood Preservation / methods*
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents
Dimethyl Sulfoxide
Female
Fetal Blood / cytology*
Gestational Age
Humans
Infant, Newborn
Leukocytes, Mononuclear / cytology*
Pregnancy
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide; EC 3.1.3.48/Antigens, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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