| The effect of pre- and probiotics on the colonic ammonia metabolism in humans as measured by lactose-[(15)N(2)]ureide. | |
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MedLine Citation:
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PMID: 20683456 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND: The evaluation of ammonia detoxification by pre- and probiotics by means of colonic lactose-[(15)N(2)]ureide ((15)N-LU) degradation is of great interest both scientifically and in terms of nutrition physiology. OBJECTIVE: Pre- and probiotics were supplemented in healthy adults to evaluate the effect of the ammonia metabolism in the human colon by means of (15)N-LU.Methods:A total of 14 participants aged 20-28 years daily received a regular diet either without (no treatment) or with supplementation of 30 g fibre of potatoes (FPs), 30 g wrinkle pea starch (WPS, resistant starch content: 12 and 70%, respectively) and 375 g Lactobacillus acidophilus (LC1) yoghurt, over a 10-day period in a randomised order. After 1 week, 5.7 mg/kg body weight (15)N-LU was administered together with breakfast. A venous blood sample was taken after 6 h. Urine and faeces were collected over a period of 48 and 72 h, respectively. The (15)N abundances were measured by isotope ratio mass spectrometry. RESULTS: The mean renal (15)N-excretion differed significantly between the supplementation of FP and no treatment (32.5 versus 46.3%, P=0.034), FP and LC1 (32.5 versus 51.6%, P=0.001), and WPS and LC1 (38.5 versus 51.6%, P=0.048). The mean faecal (15)N-excretion amounted to 42.7% (no treatment), 59.7% (FP), 41.8% (WPS) and 44.0% (LC1). In comparison with no treatment, the urinary (15)NH(3)-enrichment was significantly decreased at 16 h after FP supplementation. CONCLUSION: The prebiotic intake of FP and WPS lowered the colonic generation and the renal excretion of toxic (15)NH(3), respectively, when using (15)N-LU as a xenobiotic marker. |
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Authors:
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K D Wutzke; M Lotz; C Zipprich |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-04 |
Journal Detail:
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Title: European journal of clinical nutrition Volume: 64 ISSN: 1476-5640 ISO Abbreviation: Eur J Clin Nutr Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804070 Medline TA: Eur J Clin Nutr Country: England |
Other Details:
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Languages: eng Pagination: 1215-21 Citation Subset: IM |
Affiliation:
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Research Laboratory 'Gastroenterology and Nutrition', Children's Hospital, University of Rostock, Rostock, Germany. klaus-dieter.wutzke@med.uni-rostock.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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