| The effect of photomirex on the in vitro perfused ovary of the rat. | |
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MedLine Citation:
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PMID: 9628554 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photomirex, a photodegradation product of the insecticide mirex, is an environmental contaminant that has been identified in Great Lakes fish, soil, and human adipose tissue. Because of the potential for human exposure, the present study was designed to investigate the short-term effects of photomirex on the in vitro perfused ovary of the rat. Adult Sprague-Dawley rat ovaries were isolated and perfused for a total of 6 h with Medium 199. Following a 2-h baseline period, 10(-4) M of photomirex was administered to the medium. Control ovaries received medium or DMSO (vehicle control). Significant effects of perfusion and chemical intervention were identified using lactate dehydrogenase enzyme, glucose utilization, lactate, pyruvate, and flow:pressure ratio as markers of toxicity (P < 0.05). Lactate:pyruvate ratio, glutathione, and oxygen consumption did not demonstrate significant effects. Post hoc tests showed that there were significant differences between the DMSO + photomirex group and the control group (M199) using lactate dehydrogenase as a marker of toxicity. Pyruvate concentration was also reduced significantly after perfusion with DMSO + photomirex compared to M199 only and DMSO only (P < 0.05). Histopathologic changes were not discernible by light microscopy. These results suggest that metabolic and respiratory processes of the ovary are acutely sensitive to perturbation with photomirex in the in vitro perfused rat ovary model. |
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Authors:
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E C Todoroff; M Sevcik; D C Villeneuve; W G Foster; J F Jarrell |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproductive toxicology (Elmsford, N.Y.) Volume: 12 ISSN: 0890-6238 ISO Abbreviation: Reprod. Toxicol. Publication Date: 1998 May-Jun |
Date Detail:
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Created Date: 1998-08-27 Completed Date: 1998-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8803591 Medline TA: Reprod Toxicol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 305-16 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynaecology, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Weight / drug effects Diet* Female Glucose / metabolism Glutathione / metabolism Lactic Acid / metabolism Mirex / analogs & derivatives*, pharmacokinetics, toxicity Organ Size / drug effects Ovary / drug effects* Perfusion Pyruvic Acid / metabolism Rats Rats, Sprague-Dawley Soil Pollutants / pharmacokinetics, toxicity* Tissue Distribution Water Pollutants, Chemical / pharmacokinetics, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Soil Pollutants; 0/Water Pollutants, Chemical; 127-17-3/Pyruvic Acid; 2385-85-5/Mirex; 39801-14-4/photomirex; 50-21-5/Lactic Acid; 50-99-7/Glucose; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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