| The effect of peptide absorption on PepT1 gene expression and digestive system hormones in rainbow trout (Oncorhynchus mykiss). | |
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MedLine Citation:
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PMID: 19854288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study evaluates the effect of protein source (dipeptides, free amino acids, and intact protein) on development and growth of Salmonid fish alevin. Specifically, we follow the expression of oligopeptide transporter protein PepT1 in the intestine of rainbow trout (Oncorhynchus mykiss). Fish were fed exogenously one of four diets: three formulated (lysyl-glycine dipeptide supplemented diet - PP, free lysine and glycine supplemented diet - AA, control diet with no lysine - CON) or commercial starter (Aller Futura - AF). Fish increased mean body weight 8 fold with PP- and AA-supplemented diets resulting in significantly higher weight gain than fish fed CON. Statistical analysis revealed a significant increase in relative PepT1 expression of fish fed experimental diets. Immunohistochemical staining with PepT1 antibody showed the presence of the transporter protein in the brush border membrane of the proximal intestinal enterocytes of fish from all experimental groups. Leptin immunoreactivity occurred not only in the gastric glands but also in proximal intestine and pyloric caeca of fish fed PP, AA and AF diets. Leptin immunoreactivity was also observed in hepatocyte cytoplasm and pancreatic acinar cells. Gastrin/CCK immunoreactive cells were present in the proximal intestine and pyloric caeca. |
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Authors:
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Teresa Ostaszewska; Maciej Kamaszewski; Piotr Grochowski; Konrad Dabrowski; Tiziano Verri; Erc?ment Aksakal; Iwona Szatkowska; Zuzanna Nowak; Stefan Dobosz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-22 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Volume: 155 ISSN: 1531-4332 ISO Abbreviation: Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-11-30 Completed Date: 2010-03-04 Revised Date: 2010-06-25 |
Medline Journal Info:
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Nlm Unique ID: 9806096 Medline TA: Comp Biochem Physiol A Mol Integr Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 107-14 Citation Subset: IM |
Affiliation:
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Warsaw University of Life Sciences, Division of Ichthyobiology and Fisheries, Faculty of Animal Science, Ciszewskiego 8, 02-786 Warsaw, Poland. teresa_ostaszewska@sggw.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animal Feed Animal Nutritional Physiological Phenomena Animals Cholecystokinin / genetics, metabolism Diet Digestive System / drug effects*, metabolism Gastrins / genetics, metabolism Gastrointestinal Hormones / genetics*, metabolism Gene Expression / drug effects Leptin / genetics, metabolism Oncorhynchus mykiss / genetics*, growth & development, metabolism Peptides / pharmacokinetics*, pharmacology Symporters / genetics* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Gastrins; 0/Gastrointestinal Hormones; 0/Leptin; 0/PepT1 protein; 0/Peptides; 0/Symporters; 9011-97-6/Cholecystokinin |
| Comments/Corrections | |
Erratum In:
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Comp Biochem Physiol A Mol Integr Physiol. 2010 Aug;156(4):569 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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