Document Detail

The effect of pH on silver staining of nucleolus organizer regions.
MedLine Citation:
PMID:  2440148     Owner:  NLM     Status:  MEDLINE    
The effect of pH on silver staining of the nucleolus organizer regions (NORs) of human chromosomes has been investigated between pH 6.5 and 12.0. Nonvolatile mixtures of ethanolamine and ethanolammonium nitrate replaced the ammonia of standard procedures. The optimal NOR staining obtained at pH 3.5 by the silver staining procedure of Howell and Black served as a standard; this procedure stained all ten NORs in 90% of mitoses. Similar NOR staining was found in 75% of mitoses stained at pH 11.7 or 11.8, but only in 10-15% of mitoses stained between pH 11.6 and 10.0. Between pH 10.0 and 9.0 NOR staining was incomplete, and between pH 8.5 and 6.5 there was no NOR staining.
B E Lomholt; J M Toft
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  62     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-08-10     Completed Date:  1987-08-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  101-5     Citation Subset:  IM    
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MeSH Terms
Chromosome Banding
Hydrogen-Ion Concentration
Microscopy, Phase-Contrast
Nucleolus Organizer Region / ultrastructure*
Silver Nitrate*
Staining and Labeling
Reg. No./Substance:
0/Ethanolamines; 141-43-5/Ethanolamine; 7761-88-8/Silver Nitrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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