| The effect of ouabain on water diffusion in the rat hippocampal slice measured by high resolution NMR imaging. | |
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MedLine Citation:
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PMID: 10025621 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High resolution NMR imaging of the isolated perfused rat hippocampal slice was used to quantitate ADC changes following ouabain-induced cell swelling. Hippocampal slices were studied in artificial cerebrospinal fluid and then in ouabain using a 600-MHz narrow bore spectrometer and a home-built perfusion chamber. The brain slices demonstrated biexponential diffusion behavior. After perfusion with 1 mMouabain, there was an increase in the fraction of slowly diffusing water. The ADCs of the two fractions did not change. These data support the hypothesis that the decrease in the ADC of brain water following an ischemic attack is caused by cell swelling. The relative amplitudes of the two diffusing fractions do not match the expected ratio of intracellular and extracellular fractions. This discrepancy may be principally due to the difference in T2 relaxation rates of the two compartments. |
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Authors:
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D L Buckley; J D Bui; M I Phillips; T Zelles; B A Inglis; H D Plant; S J Blackband |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 41 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-04-13 Completed Date: 1999-04-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 137-42 Citation Subset: IM |
Affiliation:
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Center for Structural Biology in the University of Florida Brain Institute, Department of Neuroscience, University of Florida, Gainesville 32610-0245, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Water / metabolism* Brain Ischemia / chemically induced, metabolism, pathology Diffusion / drug effects Disease Models, Animal Enzyme Inhibitors / toxicity* Hippocampus / drug effects, metabolism*, pathology Image Processing, Computer-Assisted* Magnetic Resonance Spectroscopy* Male Ouabain / toxicity* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS36992/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 630-60-4/Ouabain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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