Document Detail

The effect of non-esterified fatty acids on the proton-pumping cytochrome c oxidase reconstituted into liposomes.
MedLine Citation:
PMID:  2902846     Owner:  NLM     Status:  MEDLINE    
Bovine heart cytochrome c oxidase was reconstituted in phospholipid vesicles, and the effect of different non-esterified fatty acids (NEFA) was studied on its proton pump and on the proton permeability of the vesicles. Neither parameter appeared to be affected by concentrations of NEFA known to uncouple oxidative phosphorylation (10 microM). Also the permeability for K+ was not affected by them. The fatty acids caused an increase in the rate of electron transfer in the absence, but not in the presence, of uncoupler and/or valinomycin [diminution of the respiratory-control index (RCI)]. The RCI of 8.7-7.5 was decreased to about 4.5 in the presence of 0.27-10 microM-NEFA. Oleic acid was not effective at the above concentrations. Subunit III-depleted enzyme preparations gave vesicles with an RCI of about 5.5, which was decreased to 4.5 in the presence of NEFA. With both native and subunit III-depleted oxidase the RCI was never decreased to the value of 1 by NEFA, as happens with classical protonophores.
N Labonia; M Müller; A Azzi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  254     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-11-22     Completed Date:  1988-11-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
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MeSH Terms
Biological Transport / drug effects
Cell Membrane Permeability / drug effects
Electron Transport Complex IV / metabolism*
Fatty Acids, Nonesterified / pharmacology*
Liposomes / metabolism*
Membrane Potentials / drug effects
Oxygen Consumption / drug effects
Palmitic Acid
Palmitic Acids / pharmacology
Potassium / pharmacology
Proton-Translocating ATPases / metabolism
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Liposomes; 0/Palmitic Acids; 0/Protons; 57-10-3/Palmitic Acid; 7440-09-7/Potassium; EC Transport Complex IV; EC ATPases

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