Document Detail

The effect of neonatal jaundice on biotinidase activity.
MedLine Citation:
PMID:  12706308     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Jaundice is one of the most common and one of the vexing problems that can occur in newborns. A newborn screening test for biotinidase deficiency has been added to many national screening programmes. AIM: To clarify the problem of false-positive screening tests in neonates, especially in term babies, we evaluated the biotinidase activity in the serum of fullterm, premature and small-for-dates newborn infants with jaundice. METHODS: 1296 fullterms (controls N=426), 246 prematures (controls N=86) and 156 small-for-dates babies (controls N=38) aged 2-3 days with jaundice were included in the study. In jaundiced neonates and controls, 3.0 ml of blood was drawn for the evaluation of total bilirubin (t.bil), liver enzymes and biotinidase activity in the serum using a fluorimetric method. In order to test whether or not t.bil causes an artifact in the previous method, biotinidase activity was also evaluated in a number of jaundiced newborns using an HPLC method. Additionally, a preliminary in vitro experiment was carried out to test whether t.bil is an inhibitor of the enzyme. RESULTS: Biotinidase activities in the group of controls of prematures (3.30+/-1.2 mmol/min/l) and small-for-dates babies (3.34+/-0.8 mmol/min/l) were lower than those of term babies (4.99+/-1.1 mmol/min/l, p<0.001). T.bil and liver enzymes showed a statistically significant inverse correlation with biotinidase activity (p<0.001) in all the jaundiced infants of this study. Additionally, biotinidase activity, evaluated in a number of neonates with both fluorimetric and HPLC methods showed similar results. Preincubation of the serum enzyme with t.bil (>10 mg/dl) resulted in a 50% or more inhibition. CONCLUSIONS: (a) Low biotinidase activity was found in term babies, prematures and small-for-dates with jaundice. (b) The low activity of the enzyme could be due to their impaired liver function. (c) The high t.bil levels in the studied groups may play the role of an "inhibitor" of the enzyme. (d) Gestational age as well as t.bil levels should always be written on Guthrie cards for a correct evaluation of biotinidase activity.
Kleopatra H Schulpis; Stavroula Gavrili; Joanna Tjamouranis; George A Karikas; Angeliki Kapiki; Christos Costalos
Related Documents :
10389958 - Apparent life-threatening events presenting to a pediatric emergency department.
17062308 - The newborn drug development initiative workshop: summary proceedings from the neurolog...
3105818 - Kindling antagonism: a role for hindbrain norepinephrine in the development of site sup...
23569738 - Glucose-6-phosphate-dehydrogenase deficiency and its correlation with other risk factor...
9252078 - Transmission of gbv-c/hgv from drug-addicted mothers to their babies.
20064408 - Communicative development: neonate crying reflects patterns of native-language speech.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Early human development     Volume:  72     ISSN:  0378-3782     ISO Abbreviation:  Early Hum. Dev.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708381     Medline TA:  Early Hum Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  15-24     Citation Subset:  IM    
Institute of Child Health, "Aghia Sophia" Children's Hospital, 11527, Athens, Greece.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amidohydrolases / blood*
Bilirubin / blood*
Infant, Newborn
Infant, Premature / blood*
Infant, Small for Gestational Age / blood*
Jaundice, Neonatal / diagnosis,  enzymology*
Liver Function Tests
Neonatal Screening / methods
Reg. No./Substance:
635-65-4/Bilirubin; EC 3.5.-/Amidohydrolases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fetal monitoring in type 1 diabetic pregnancies.
Next Document:  Occurrence of oxygen desaturation events during preterm infant bottle feeding near discharge.