Document Detail

The effect of a mutagen (N-methyl-N-nitro-N-nitrosoguanidine) on cultured cells from adult Schistosoma japonicum.
MedLine Citation:
PMID:  16385406     Owner:  NLM     Status:  MEDLINE    
The transforming effect of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) on cultured cells from Schistosoma japonicum (S. japonicum) was studied using mono-factor and orthogonal tests. Under the influence of MNNG, cultured cells grew well, and cell survival time was more than 246 days in low-serum medium. When treated with 3 mug/ml MNNG for 48 h, the number of dividing cells increased significantly as determined by bright-field and scanning electron microscopy (SEM). Under these conditions, abundant microvilli, ruffles, microridges, papillae and blebs were observed on the surface of the induced cells. Treatment with MNNG may overcome existing limitations to get continually proliferating schistosome cells and open the possibility to immortalize isolated cells.
Zhenping Ming; Huifen Dong; Qinping Zhong; Christoph G Grevelding; Mingsen Jiang
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Publication Detail:
Type:  Journal Article     Date:  2005-12-30
Journal Detail:
Title:  Parasitology research     Volume:  98     ISSN:  0932-0113     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-22     Completed Date:  2007-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  430-7     Citation Subset:  IM    
Department of Medical Parasitology and Research Laboratory of Schistosomiasis, Wuhan University School of Medicine, Wuhan, Hubei Province, 430071, People's Republic of China.
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MeSH Terms
Cell Survival
Cell Transformation, Neoplastic*
Cells, Cultured
Dose-Response Relationship, Drug
Methylnitronitrosoguanidine / pharmacology*
Microscopy, Electron, Scanning
Microvilli / ultrastructure
Mutagens / pharmacology*
Schistosoma japonicum / cytology*,  drug effects*,  ultrastructure
Reg. No./Substance:
0/Mutagens; 70-25-7/Methylnitronitrosoguanidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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