Document Detail


The effect of microbial-nutrient interaction on the immune system of young chicks following early probiotic and organic acid administration.
MedLine Citation:
PMID:  22247115     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The combined effects of probiotics (Lactobacillus acidophilus, Lactobacillus casei, Streptococcus faecium, and Saccharomyces cerevisae) and organic acids (sorbic and citric acid) on intestinal morphology and expression of immune-related genes were investigated. Day-old chicks were randomly assigned to 1 of 3 groups: birds not receiving probiotic or organic acids (control; T1), birds receiving an oral combination (1 g/L in water) of 10(8) cfu/g of each of the aforementioned probiotics and organic acids for 7 (T2) or 14 d (T3). Each group was divided into 5 replicate pens of 20 birds each, and 5 birds from each group (1 from each pen) were sacrificed on d 11 and 22. Intestinal sections were collected for histological assessment, and reverse transcriptase polymerase chain reaction (RT-PCR) analysis was used to assess defensin and cathelicidins expression. Quantitative real-time PCR was used to assess toll-like receptors (TLR) and cytokine expression. Duodenal villus height was greater in T2 and T3 at d 11 (P ≤ 0.036) and 22 (P ≤ 0.015) compared to T1. At d 11, duodenal goblet cell/unit area was lower in T3, while it was greater in T2 compared to T1 in the jejunum (P = 0.009). Ileal goblet cell/unit area was greater in T3 at d22 compared to T1 (P < 0.001). Avian beta-defensin (AvBD)-3 was expressed in all tissues except the bursa of T3 birds at d 11, and TLR-2 was down regulated in the cecal tonsil of birds in T2 and T3 at d 11 compared to T1 (P = 0.020 and 0.003, respectively). Expression of IL-12p35 in the ileum at d 11 was down regulated in T2 and T3 compared to T1 (P = 0.030 and 0.012, respectively). Lower expression of INF-γ was observed in the ileum in T3 compared to T1 at d 11 (P = 0.047). Ileal IL-6 and IL-10 and cecal tonsil interferon-gamma (INF-γ) expressions were greater T2 at d 22 (P ≤ 0.047) than T1. In conclusion, supplementation of combined probiotics and organic acids resulted in inconsistent gut morphology associated responses, and avian beta-defensins and cathelicidins expression were not associated with combined probiotics and organic acids supplementation. Birds supplemented with combined probiotics and organic acids for 7 d showing similar responses in TLR-2, IL12p40, and IFN-γ compared to those supplemented for 14 d indicates that shorter periods of supplementation might be enough to elicit beneficial responses.
Authors:
J C Rodríguez-Lecompte; A Yitbarek; J Brady; S Sharif; M D Cavanagh; G Crow; W Guenter; J D House; G Camelo-Jaimes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-13
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
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