Document Detail

The effect of mechanical strain on protease production by keratinocytes.
MedLine Citation:
PMID:  18067479     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Intact skin is under constant tension, transmitted from the underlying dermis, but when tension is lost (i.e. upon wounding) protease activity is upregulated. OBJECTIVES: To investigate the effect of mechanical strain on protease production by both normal and transformed keratinocytes in vitro. METHODS: Keratinocytes were seeded on to membranes precoated with either type I or type IV collagen. After 48 h medium was replaced with serum-free medium and mechanical strain was applied. RESULTS: Mechanical strain resulted in decreased urokinase-type plasminogen activator (uPA) production by normal human keratinocytes (P<0.05) but increased production by transformed keratinocytes (P<0.05) cultured on type I and type IV collagen. CONCLUSIONS: Differential production of uPA by normal and transformed keratinocytes is relevant in the context of normal function, wound healing and tumorigenesis.
N Bhadal; I B Wall; S R Porter; S Broad; G E Lindahl; S Whawell; M P Lewis
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Publication Detail:
Type:  Journal Article     Date:  2007-12-07
Journal Detail:
Title:  The British journal of dermatology     Volume:  158     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-04-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  396-8     Citation Subset:  IM    
UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
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MeSH Terms
Cells, Cultured / metabolism
Keratinocytes / metabolism*
Skin / metabolism*
Stress, Mechanical
Urokinase-Type Plasminogen Activator / metabolism*
Wound Healing / physiology
Reg. No./Substance:
EC Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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