Document Detail

The effect of macrophages on the metabolism of glomerular cells: preliminary studies.
MedLine Citation:
PMID:  6219219     Owner:  NLM     Status:  MEDLINE    
Human glomerular epithelial and mesangial cells were grown in vitro and shown to have distinctive morphologic and functional characteristics. Glomerular epithelial cells or mesangial cells cultured in wells of flat-bottom microtiter plates were treated for 4 hr with dialyzed macrophage supernatants obtained from cultures of mouse peritoneal macrophages or human peripheral monocytes. DNA, RNA, and protein synthesis were evaluated by incorporation of radioactive precursors. Macrophage supernatants stimulated RNA and protein synthesis in epithelial cells but failed to stimulate DNA synthesis. The macrophage factor(s) showed a dose-response activity, was nondialyzable, was destroyed by freezing and thawing, and did not seem to be species specific. In contrast to the results obtained with glomerular epithelial cells, mesangial cell DNA synthesis was stimulated by macrophage supernatants. The observed metabolic effects of macrophage products on glomerular cells in vitro are consistent with observations of in vivo glomerular response to injury in which epithelial cells may be activated to form new basement membrane while mesangial cells may respond by proliferating. These data further support the theory of macrophage involvement in the pathology of glomerulonephritis.
C M Wagner; D O Lucas; R B Nagle
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Reticuloendothelial Society     Volume:  33     ISSN:  0033-6890     ISO Abbreviation:  J Reticuloendothel Soc     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0206462     Medline TA:  J Reticuloendothel Soc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-107     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
DNA / biosynthesis
Epithelium / metabolism
Kidney Glomerulus / cytology,  metabolism*,  ultrastructure
Macrophages / physiology*
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Monocytes / physiology
Receptors, Complement / physiology
Receptors, Complement 3b
Grant Support
Reg. No./Substance:
0/Receptors, Complement; 0/Receptors, Complement 3b; 9007-49-2/DNA

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