| The effect of macrophages on the metabolism of glomerular cells: preliminary studies. | |
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MedLine Citation:
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PMID: 6219219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human glomerular epithelial and mesangial cells were grown in vitro and shown to have distinctive morphologic and functional characteristics. Glomerular epithelial cells or mesangial cells cultured in wells of flat-bottom microtiter plates were treated for 4 hr with dialyzed macrophage supernatants obtained from cultures of mouse peritoneal macrophages or human peripheral monocytes. DNA, RNA, and protein synthesis were evaluated by incorporation of radioactive precursors. Macrophage supernatants stimulated RNA and protein synthesis in epithelial cells but failed to stimulate DNA synthesis. The macrophage factor(s) showed a dose-response activity, was nondialyzable, was destroyed by freezing and thawing, and did not seem to be species specific. In contrast to the results obtained with glomerular epithelial cells, mesangial cell DNA synthesis was stimulated by macrophage supernatants. The observed metabolic effects of macrophage products on glomerular cells in vitro are consistent with observations of in vivo glomerular response to injury in which epithelial cells may be activated to form new basement membrane while mesangial cells may respond by proliferating. These data further support the theory of macrophage involvement in the pathology of glomerulonephritis. |
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Authors:
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C M Wagner; D O Lucas; R B Nagle |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of the Reticuloendothelial Society Volume: 33 ISSN: 0033-6890 ISO Abbreviation: J Reticuloendothel Soc Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-04-15 Completed Date: 1983-04-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0206462 Medline TA: J Reticuloendothel Soc Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 93-107 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured DNA / biosynthesis Epithelium / metabolism Female Humans Kidney Glomerulus / cytology, metabolism*, ultrastructure Macrophages / physiology* Mice Mice, Inbred BALB C Microscopy, Electron, Scanning Monocytes / physiology Rabbits Receptors, Complement / physiology Receptors, Complement 3b |
| Grant Support | |
ID/Acronym/Agency:
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SO7RR05675-11/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Complement; 0/Receptors, Complement 3b; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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