Document Detail


The effect of macrophages on the metabolism of glomerular cells: preliminary studies.
MedLine Citation:
PMID:  6219219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human glomerular epithelial and mesangial cells were grown in vitro and shown to have distinctive morphologic and functional characteristics. Glomerular epithelial cells or mesangial cells cultured in wells of flat-bottom microtiter plates were treated for 4 hr with dialyzed macrophage supernatants obtained from cultures of mouse peritoneal macrophages or human peripheral monocytes. DNA, RNA, and protein synthesis were evaluated by incorporation of radioactive precursors. Macrophage supernatants stimulated RNA and protein synthesis in epithelial cells but failed to stimulate DNA synthesis. The macrophage factor(s) showed a dose-response activity, was nondialyzable, was destroyed by freezing and thawing, and did not seem to be species specific. In contrast to the results obtained with glomerular epithelial cells, mesangial cell DNA synthesis was stimulated by macrophage supernatants. The observed metabolic effects of macrophage products on glomerular cells in vitro are consistent with observations of in vivo glomerular response to injury in which epithelial cells may be activated to form new basement membrane while mesangial cells may respond by proliferating. These data further support the theory of macrophage involvement in the pathology of glomerulonephritis.
Authors:
C M Wagner; D O Lucas; R B Nagle
Related Documents :
2510469 - Cyclooxygenase inhibitors enhance cell growth in an osteoblastic cell line, mc3t3-e1.
3109729 - Effect of prostaglandin e2-producing nonmetastatic lewis lung carcinoma cells on the mi...
11854809 - Effect of misoprostol on mesangial cell growth and collagen metabolism.
8807589 - Cell apoptosis and proliferation in experimental chronic obstructive uropathy.
7529619 - Cell culture from rat renal glomeruli.
8345409 - Immune complex degradation by cultured rat mesangial cells.
5415239 - Electrical coupling between embryonic cells by way of extracellular space and specializ...
1257749 - Marine oscillatoria (trichodesmium): explanation for aerobic nitrogen fixation without ...
3993599 - Dynamic histology of the antral epithelium in the mouse stomach: iv. ultrastructure and...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Reticuloendothelial Society     Volume:  33     ISSN:  0033-6890     ISO Abbreviation:  J Reticuloendothel Soc     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0206462     Medline TA:  J Reticuloendothel Soc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-107     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
DNA / biosynthesis
Epithelium / metabolism
Female
Humans
Kidney Glomerulus / cytology,  metabolism*,  ultrastructure
Macrophages / physiology*
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Monocytes / physiology
Rabbits
Receptors, Complement / physiology
Receptors, Complement 3b
Grant Support
ID/Acronym/Agency:
SO7RR05675-11/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Complement; 0/Receptors, Complement 3b; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enzyme variation among clones of Trypanosoma cruzi.
Next Document:  Evaluation of T cell subsets with monoclonal antibodies in synovial fluid in rheumatoid arthritis.