Document Detail

The effect of lycopene on cell growth and oxidative DNA damage of Hep3B human hepatoma cells.
MedLine Citation:
PMID:  16410635     Owner:  NLM     Status:  MEDLINE    
Lycopene, the predominant carotenoid in tomatoes and tomato-based foods, is reported to protect against various cancers, especially prostate cancer. We investigated the effect of lycopene on DNA damage and cell growth inhibition in the Hep3B human hepatoma cell line. Lycopene was analyzed by HPLC, and cell proliferation was determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. A final lycopene concentration of 0.1-50 microM was added to cells plated in 96-well plates. After a 24-hr incubation, cell viability was measured as absorbance at 570 nm after the MTT assay. The effects of lycopene on cell cycle progression were investigated with flow cytometry. Lycopene induced G0/G1 arrest and S phase block. Oxidative DNA damage was determined by the Comet (single-cell gel electrophoresis) assay. Lycopene inhibited cell growth in a dose-dependent manner. Cell growth was inhibited 20% at 0.2 microM lycopene and 40% at 50 microM lycopene after a 24-hr incubation. In the Comet assay, lycopene-treated cells showed less DNA damage than did placebo-treated cells. The inhibition of Hep3B cell growth in this study demonstrates the antitumor properties of lycopene.
Yun Ok Park; Eun-Sun Hwang; Tae Wha Moon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioFactors (Oxford, England)     Volume:  23     ISSN:  0951-6433     ISO Abbreviation:  Biofactors     Publication Date:  2005  
Date Detail:
Created Date:  2006-01-13     Completed Date:  2006-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8807441     Medline TA:  Biofactors     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  129-39     Citation Subset:  IM    
Department of Food Science and Biotechnology, School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Korea.
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MeSH Terms
Antioxidants / pharmacology*
Carcinoma, Hepatocellular
Carotenoids / analysis,  pharmacology*
Cell Cycle / drug effects
Cell Division / drug effects*
Cell Line, Tumor
Chromatography, High Pressure Liquid
DNA Damage / drug effects*
Flow Cytometry
G0 Phase / drug effects
G1 Phase / drug effects
Liver Neoplasms
S Phase / drug effects
Reg. No./Substance:
0/Antioxidants; 36-88-4/Carotenoids; 502-65-8/lycopene

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