Document Detail


The effect of losartan treatment on the response of diabetic cardiomyocytes to ATP depletion.
MedLine Citation:
PMID:  21078391     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The present work aimed to investigate the effect of losartan treatment of healthy and diabetic rats on cardiomyocyte response to ATP depletion. Cells were isolated from normoglycemic (N) and streptozotocin-injected (55mg/kg) rats (D) treated or not treated with losartan (20mg/kg/day in the drinking water; NL and DL, respectively) for 3 weeks. In each group of cells, enzyme activities such as glucose-6-phosphate (G6PDH) and glycerol-3-phosphate dehydrogenases (G3PDH), lactate/pyruvate, glycogen levels and citrate synthase were measured as an index of glycolytic dysregulation and mitochondrial mass, respectively. Cells were then challenged with NaCN (2mM) in glucose-free Tyrode solution (metabolic intoxication, MI), a protocol to study ischemia at cell level. Under these conditions, the time to contractile failure up to rigor-type hyper-contracture in field-stimulated cells and K(ATP) current activation by patch-clamp recordings were measured. In comparison with N and NL, D cells presented higher G6PDH and cytoplasmic G3PDH activities, lactate/pyruvate, glycogen content but similar levels of citrate synthase, and decay time of contraction. When subjected to MI, D cells showed delayed activation of the K(ATP) current (25.7±7.1min; p<0.001 vs. N and NL), increased time to contractile failure and rigor-type hyper-contracture (p<0.001 vs. N and NL). In cells from DL rats both functional (time to rigor and to K(ATP) current activation) and metabolic parameters, approached values similar to those measured in N and NL cells. These results demonstrate that diabetic cardiomyocytes from rats treated with losartan, maintain the capacity to respond promptly to ATP depletion reaching contractile failure, rigor-type hypercontracture and K(ATP) opening with a similar timing of N cells.
Authors:
Chiara Alfarano; Silvia Suffredini; Ornella Fantappiè; Alessandro Mugelli; Elisabetta Cerbai; Maria Elena Manni; Laura Raimondi
Publication Detail:
Type:  Journal Article     Date:  2010-11-13
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  63     ISSN:  1096-1186     ISO Abbreviation:  Pharmacol. Res.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Dept. of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor...
Next Document:  Using Sculptor and Situs for simultaneous assembly of atomic components into low-resolution shapes.