| The effect of long-term storage on measured plasma lactate concentrations and prospective lactate results from a multicenter trial of antiretroviral therapy. | |
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MedLine Citation:
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PMID: 16176175 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasma lactate measurements are typically performed in real time, limiting their usefulness in multicenter or longitudinal studies. To determine the stability of lactate specimens, blood was drawn in sodium fluoride/potassium oxalate tubes from 13 volunteers before and after 5 min of handgrip exercise to intentionally increase lactate concentrations. Plasma was stored at -70 degrees C. Aliquots were assayed in real time and after 1, 3, 6, 9, 12, 18, and 24 months. Real-time lactate concentrations measured at baseline ranged from 0.52 to 2.23 mmol/L before and from 2.91 to 11.04 mmol/L after handgrip exercise. Using a linear mixed model, the estimated change from baseline at month 24 was 1.67% (95% confidence interval, -0.70% to 4.03%) for pre-exercise samples and 0.39% (95% CI, -1.13% to 1.91%) for post-exercise samples. Stored serial specimens from 232 HIV-infected subjects in a multicenter trial of antiretroviral therapy were also assayed centrally. Among those, median plasma lactate increased from baseline to 64 weeks by 0.4 mmol/L with zidovudine+lamivudine treatment and by 0.6 mmol/L with didanosine+stavudine (each p<0.001 from baseline; p=0.04 for difference between groups over time). When performed as in this study, frozen storage with central batch lactate analysis is appropriate for prospectively collected samples in multicenter trials. |
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Authors:
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Michael P Dubé; Douglas W Kitch; Robert A Parker; Beverly L Alston-Smith; Kathleen Mulligan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 43 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-09-22 Completed Date: 2005-12-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 947-52 Citation Subset: IM |
Affiliation:
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Department of Medicine and Division of Infectious Diseases, Indiana University School of Medicine, Wishard Memorial Hospital, 1001 W. 10th Street, Suite OPW-430, Indianapolis, IN 46202, USA. mpdube@iupui.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis, Lactic
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blood Anti-HIV Agents / therapeutic use* Blood Preservation / methods Blood Specimen Collection / methods* Didanosine / therapeutic use HIV Infections / blood*, drug therapy*, virology Humans Lactates / blood* Lamivudine / therapeutic use Multicenter Studies as Topic Oxalates Randomized Controlled Trials as Topic Reverse Transcriptase Inhibitors / therapeutic use Sodium Fluoride Specimen Handling* Stavudine / therapeutic use Time Factors Zidovudine / therapeutic use |
| Grant Support | |
ID/Acronym/Agency:
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AI 25859/AI/NIAID NIH HHS; AI 27663/AI/NIAID NIH HHS; AI 38855/AI/NIAID NIH HHS; AI 38858/AI/NIAID NIH HHS; RR 00083/RR/NCRR NIH HHS; RR 00750/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-HIV Agents; 0/Lactates; 0/Oxalates; 0/Reverse Transcriptase Inhibitors; 134678-17-4/Lamivudine; 30516-87-1/Zidovudine; 3056-17-5/Stavudine; 69655-05-6/Didanosine; 7681-49-4/Sodium Fluoride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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