Document Detail

The effect of lipid molecular packing stress on cationic liposome-induced rabbit erythrocyte fusion.
MedLine Citation:
PMID:  9030217     Owner:  NLM     Status:  MEDLINE    
The effect of curvature stress on the efficiency of cationic liposome-induced fusion between rabbit erythrocytes was studied. Multilamellar cationic liposomes containing 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and different PEs (1,2-dilnoleoyl-sn-glycero-3-phosphoethanolamine (dilin-PE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), and lysophosphatidylethanolamine, egg (lyso-PE)) were used to induce cell-cell fusion. It was found that high cell-cell fusion yield (FY) of about 50% could be achieved in sucrose solution by using cationic liposomes containing 50% DOTAP. Cell-cell fusion was assayed by shape criterion and was verified by fluorescence microscopy, using a membrane dye mixing method. The curvature stress, as a result of mixing unsaturated PEs in cationic liposomes, had a significant effect on cell-cell FY which increased with the degree of acyl chain unsaturation, in the order dilin-PE > DOPE > POPE > lyso-PE. Replacement of dilin-PE, DOPE, or POPE by lyso-PE gradually in cationic liposomes lowered the cell-cell FY from 50% to 1%. Furthermore, cationic liposome induced cell lysis, and fusion between cationic liposomes and cells, as assayed by the N-(lissamine rhodamine B sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt and N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rh-PE/NBD-PE) energy transfer method, followed the same order as that for cell-cell fusion. Fusion between the negatively charged PS liposomes and cationic liposomes also followed the same order. The electric double layer screening by electrolytes in NaCl-containing solution and phosphate buffered saline (PBS) was found to reduce the cell-cell FY and cell lysis. These findings suggest that liposome-induced cell-cell fusion was achieved by cationic liposomes serving as fusion-bridges among cells.
L H Li; S W Hui
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1323     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-16     Citation Subset:  IM    
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
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MeSH Terms
Cell Fusion / drug effects*,  physiology*
Erythrocytes / chemistry*,  drug effects*
Fatty Acids, Monounsaturated / chemistry
Lipids / chemistry*
Liposomes / chemistry*,  pharmacology*
Lysophospholipids / chemistry
Membrane Fusion
Phosphatidylethanolamines / chemistry
Quaternary Ammonium Compounds / chemistry
Static Electricity
Grant Support
Reg. No./Substance:
0/Cations; 0/Fatty Acids, Monounsaturated; 0/Lipids; 0/Liposomes; 0/Lysophospholipids; 0/Phosphatidylethanolamines; 0/Quaternary Ammonium Compounds; 0/lysophosphatidylethanolamine; 10015-88-0/1-palmitoyl-2-oleoylphosphatidylethanolamine; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 76391-83-8/1,2-dielaidoylphosphatidylethanolamine

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