| The effect of ligands on the uptake of iron by cells in culture. | |
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MedLine Citation:
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PMID: 1725507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Uptake of iron by a mammalian epithelial cell line (CNCM I-221) was shown to be dependent on the nature of the iron complex. Iron uptake was demonstrated by cytochemical staining and determination of redox-reactive iron in cell lysates. Three classes of ligands were investigated: (i) low molecular weight hydrophilic compounds, represented by ethylenediamine-tetraacetic acid (EDTA) and other charged ligands such as adenosine phosphates (ATP, ADP, AMP) and diethylenetriaminepentaacetic acid (DTPA), (2) low-molecular weight lipophilic ligands such as 8-hydroxyquinoline (8-HQ) and (3) a high molecular mass ligand, dextran. Iron complexed to 8-HQ accumulated intracellularly, the uptake rate of iron being 4.16 fmoles cell-1 h-1 of exposure at 37 degrees C or 3.86 fmoles cell-1 h-1 at 4 degrees C. Iron-dextran was endocytosed and retained in phagosomes. The uptake rate of iron following exposure to iron dextrans was found to be 5.6 fmoles cell-1 h-1 of exposure at 37 degrees C. In contrast to iron/8-HQ, uptake of iron dextran by cells was inhibited at 4 degrees C. Iron complexed to low molecular weight hydrophilic ligands was not taken up by cells. Cytotoxicity was measured by reduction of plating efficiency or tritiated thymidine incorporation. These tests showed that toxic effects of added iron were demonstrable only in cells exposed to the complex with 8-HQ. |
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Authors:
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S K Jonas; P A Riley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biochemistry and function Volume: 9 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1992-05-18 Completed Date: 1992-05-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 245-53 Citation Subset: IM |
Affiliation:
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Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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pharmacology Adenosine Triphosphate / pharmacology Animals Biological Transport Cell Division / drug effects Cell Line Cell Survival / drug effects Deferoxamine / pharmacology Dextrans / metabolism Epithelial Cells Ferrous Compounds / metabolism*, pharmacology Ligands Oxyquinoline / pharmacology Pentetic Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Ferrous Compounds; 0/Ligands; 148-24-3/Oxyquinoline; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 67-43-6/Pentetic Acid; 70-51-9/Deferoxamine; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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