Document Detail

The effect of lectins on Cryptosporidium parvum oocyst in vitro attachment to host cells.
MedLine Citation:
PMID:  16629306     Owner:  NLM     Status:  MEDLINE    
The influence of lectins on Cryptosporidium parvum oocyst agglutination and on attachment to both fixed Madin Darby Canine Kidney (MDCK) cells and fixed HCT-8 (human colorectal epithelial) cells was examined. Oocyst cell wall characteristics were examined by transmission electron microscopy. Lectin-free oocysts were shown to adhere equally to both MDCK cells and HCT-8 cells. In MDCK cells, the addition of 1-25 microg/ml Codium fragile lectin, 10 microg/ml Maclura pomifera lectin, 10 microg/ml Helix pomatia lectin, and 10-200 microg/ml Artocarpus integrifolia lectin significantly increased attachment to at least 1 of the cell cultures as compared to oocysts incubated without any lectin. The lectin-enhanced attachment was reversed by co-incubation of lectin treated-oocysts with 250 mM of each specific sugar (for a given lectin). In agglutination assays, concentrations as low as 0.5 microg/ml of C. fragile, M. pomifera, and A. integrifolia lectin agglutinated oocysts within 60 min. Finally, in TEM samples, colloidal gold conjugated-lectins from A. integrifolia, C. fragile, H. pomatia, and M. pomifera attached to oocysts, and this could be competitively inhibited by a lectin-specific sugar. This suggests that C. parvum oocysts are highly reactive to N-acetyl galactosamine-binding lectins and that the presence of N-acetyl-galactosamine containing molecules on oocysts can potentially help in oocyst attachment to host cells.
Barry Stein; Larry Stover; Ashley Gillem; Katherine Winters; John H Leet; Christian Chauret
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of parasitology     Volume:  92     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-04-24     Completed Date:  2006-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Indiana University Kokomo, 2300 South Washington Street, Kokomo, Indiana 46904-9003, USA.
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MeSH Terms
Agglutination / drug effects
Agglutination Tests / methods
Carbohydrate Metabolism
Cell Adhesion / drug effects
Cell Line, Tumor
Cryptosporidium parvum / drug effects*,  physiology,  ultrastructure
Glycosylation / drug effects
Gold Colloid / metabolism
Lectins / metabolism,  pharmacology*
Mice, Inbred BALB C
Microscopy, Electron, Transmission / methods
Oocysts / drug effects,  physiology,  ultrastructure
Time Factors
Reg. No./Substance:
0/Gold Colloid; 0/Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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