| The effect of lectins on Cryptosporidium parvum oocyst in vitro attachment to host cells. | |
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MedLine Citation:
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PMID: 16629306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of lectins on Cryptosporidium parvum oocyst agglutination and on attachment to both fixed Madin Darby Canine Kidney (MDCK) cells and fixed HCT-8 (human colorectal epithelial) cells was examined. Oocyst cell wall characteristics were examined by transmission electron microscopy. Lectin-free oocysts were shown to adhere equally to both MDCK cells and HCT-8 cells. In MDCK cells, the addition of 1-25 microg/ml Codium fragile lectin, 10 microg/ml Maclura pomifera lectin, 10 microg/ml Helix pomatia lectin, and 10-200 microg/ml Artocarpus integrifolia lectin significantly increased attachment to at least 1 of the cell cultures as compared to oocysts incubated without any lectin. The lectin-enhanced attachment was reversed by co-incubation of lectin treated-oocysts with 250 mM of each specific sugar (for a given lectin). In agglutination assays, concentrations as low as 0.5 microg/ml of C. fragile, M. pomifera, and A. integrifolia lectin agglutinated oocysts within 60 min. Finally, in TEM samples, colloidal gold conjugated-lectins from A. integrifolia, C. fragile, H. pomatia, and M. pomifera attached to oocysts, and this could be competitively inhibited by a lectin-specific sugar. This suggests that C. parvum oocysts are highly reactive to N-acetyl galactosamine-binding lectins and that the presence of N-acetyl-galactosamine containing molecules on oocysts can potentially help in oocyst attachment to host cells. |
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Authors:
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Barry Stein; Larry Stover; Ashley Gillem; Katherine Winters; John H Leet; Christian Chauret |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of parasitology Volume: 92 ISSN: 0022-3395 ISO Abbreviation: J. Parasitol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-04-24 Completed Date: 2006-05-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7803124 Medline TA: J Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Indiana University Kokomo, 2300 South Washington Street, Kokomo, Indiana 46904-9003, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agglutination
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drug effects Agglutination Tests / methods Animals Carbohydrate Metabolism Cell Adhesion / drug effects Cell Line, Tumor Cryptosporidium parvum / drug effects*, physiology, ultrastructure Dogs Glycosylation / drug effects Gold Colloid / metabolism Humans Lectins / metabolism, pharmacology* Mice Mice, Inbred BALB C Microscopy, Electron, Transmission / methods Oocysts / drug effects, physiology, ultrastructure Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Gold Colloid; 0/Lectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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