| The effect of intravenous insulin infusion on renal blood flow in conscious sheep is partially mediated by nitric oxide but not by prostaglandins. | |
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MedLine Citation:
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PMID: 22064745 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To test the effect of insulin on renal perfusion and the participation of nitric oxide and prostaglandins as mediators of this response, renal blood flow (RBF) was measured in sheep (n = 8) implanted with ultrasonic flowprobes around renal arteries and with a systemic arterial pressure (SAP, n = 4) telemetry device. Three protocols were performed: 1) RBF and SAP recorded (0800 to 1800 h) in fed and fasted sheep where the latter received i.v. infusions (0.5 mL/min) of insulin at 2 or 6 mU/(kg•min); 2) fasted sheep received i.v. infusions of either an inhibitor of the nitric oxide synthesis (N(G)-nitro-L-arginine methyl ester, L-NAME) alone [0.22 mg/(kg•min), 1000 to 1200 h] or L-NAME (1000 to 1200 h) + insulin during the second hour (6 mU/(kg•min), 1100 to 1200 h); and 3) the same protocol as in protocol 2, substituting L-NAME with ketoprofen [0.2 mg/(kg•min)], a cyclooxygenase inhibitor. In all protocols, plasma insulin and glucose were determined. During insulin administration, euglycemia was maintained and hypokalemia prevented by infusing glucose and KCl solutions. After the onset of meals, a long-lasting 18% rise in RBF and a 48% insulin increase were observed (P < 0.05), without changes in SAP. Low- and high-dose insulin infusions increased RBF by 19 and 40%, respectively (P < 0.05). As after meals, the rises in RBF lasted longer than the insulin increase (P < 0.05). The L-NAME infusion decreased RBF by 15% (P < 0.05); when insulin was added, RBF rose to pre-infusion values. Ketoprofen decreased RBF by 9% (P < 0.05); when insulin was added, RBF rose to 13% above pre-infusion values (P < 0.05). In no case were SAP or glucose modifications noted during the RBF changes. In conclusion, insulin infusion mimics the meal-dependent rise in RBF, independent of SAP, and lasts longer than the blood insulin plateau. The RBF increase induced by insulin was only partially prevented by L-NAME. Ketoprofen failed to prevent the insulin-dependent RBF increase. Both facts suggested that complementary vasodilatatory agents accounted for the insulin effect on sheep renal hemodynamics. |
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Authors:
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I Tebot; J-M Bonnet; C Paquet; J-Y Ayoub; S M Da Silva; V Louzier; A Cirio |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-7 |
Journal Detail:
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Title: Journal of animal science Volume: - ISSN: 1525-3163 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Departamento de Fisiología, Facultad de Veterinaria, Universidad de la República, Montevideo 11600, Uruguay. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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