Document Detail


The effect of intraperitoneally administered microcystin-LR on the gastrointestinal tract of Balb/c mice.
MedLine Citation:
PMID:  15033322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microcystin-LR (MCLR) has been associated with the development of gastrointestinal complaints in people ingesting cyanobacterial bloom contaminated water. The symptoms usually present a day or two after exposure raising questions as to the toxic effects of MCLR on the gastrointestinal tract. This study investigated the apoptotic effect of ip administered MCLR over time on the duodenum, jejenum and ileum of mice receiving a single 75% LD(50) dose. The apoptotic index was significantly raised in all sections at 8 h post exposure (pe) and continued to rise for the 16, 24 and 32 h pe groups, while the glycogen levels were normal at 24 h pe. The duodenum exhibited the most significant increase in apoptotic index overall, followed by the jejenum and ileum. Immunohistochemistry indicated the presence of MCLR in the lamina propria suggesting a role for MCLR in the induction of apoptosis in the GIT of mice exposed to a single sublethal dose of MCLR.
Authors:
Nicolette Botha; Maryna van de Venter; Tim G Downing; Enid G Shephard; Michelle M Gehringer
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  43     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-22     Completed Date:  2004-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  251-4     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Microbiology, University of Port Elizabeth, P.O. Box 1600, Port Elizabeth 6000, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Bacterial Toxins / administration & dosage,  pharmacology*
Cyanobacteria
Duodenum / drug effects
Female
Ileum / drug effects
Immunohistochemistry
Injections, Intraperitoneal
Intestine, Small / drug effects*
Jejunum / drug effects
Mice
Mice, Inbred BALB C
Microcystins
Peptides, Cyclic / administration & dosage,  pharmacology*
Specific Pathogen-Free Organisms
Water Microbiology
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Microcystins; 0/Peptides, Cyclic; 101043-37-2/cyanoginosin LR

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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