Document Detail


The effect of interstitial air on the in vitro thrombogenicity of ePTFE vascular grafts.
MedLine Citation:
PMID:  11539826     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
Gas trapped in the interstices of the biomaterials used for vascular prostheses causes thrombosis, and the process of eliminating this gas is known as denucleation. An apparatus was developed for testing in the in vitro effects of denucleation on 4 mm I.D. expanded polytetrafluoroethylene (ePTFE) Vitagraft (Johnson and Johnson). The apparatus was designed to ensure that neither the blood nor the grafts came in contact with air. Blood from a single donor was incubated with control and denucleated grafts for 5, 10, 15, 20, and 30 minutes. The thrombus volume in the graft lumen was measured with a computer assisted videometric system. Little thrombus formed by 5 or 10 minutes, but there was less thrombus in the denucleated graft than in the control graft at all times. The differences were statistically significant at 15 and 20 minutes (p < 0.05). Denucleation nearly doubled the thrombus formation time. Thrombus was more adherent to denucleated grafts than to control grafts. These results are consistent with in vivo observations in the rat where denucleation decreased thrombus formation and increased patency duration.
Authors:
S N Rashid; H G Clark; R D Vann; W A Gerth; L A Palmos; E M Mikat
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioactive and compatible polymers     Volume:  7     ISSN:  0883-9115     ISO Abbreviation:  J Bioact Compat Polym     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1997-10-10     Completed Date:  1997-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8710961     Medline TA:  J Bioact Compat Polym     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  54-64     Citation Subset:  S    
Affiliation:
Department of Biomedical Engineering, Duke University, Durham, NC 27707, USA.
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MeSH Terms
Descriptor/Qualifier:
Air
Biocompatible Materials / adverse effects*
Blood Vessel Prosthesis / adverse effects*
Embolism, Air / etiology,  physiopathology*,  prevention & control
Extracellular Space
Graft Occlusion, Vascular / etiology,  physiopathology,  prevention & control
Humans
Polytetrafluoroethylene / adverse effects*
Thrombosis / blood,  etiology,  prevention & control*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 9002-84-0/Polytetrafluoroethylene
Investigator
Investigator/Affiliation:
R D Vann / Duke U, Durham, NC, Dept Anesthesiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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