Document Detail

The effect of interleukin-17 on the proliferation and invasion of JEG-3 human choriocarcinoma cells.
MedLine Citation:
PMID:  16533341     Owner:  NLM     Status:  MEDLINE    
PROBLEM: As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion. METHOD OF STUDY: JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. RESULTS: IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells. CONCLUSION: In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.
Sutatip Pongcharoen; Pannika Niumsup; Donruedee Sanguansermsri; Kwansuda Supalap; Puntharee Butkhamchot
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  55     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-14     Completed Date:  2006-05-24     Revised Date:  2008-05-06    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  291-300     Citation Subset:  IM    
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
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MeSH Terms
Cell Line, Tumor
Cell Proliferation*
Choriocarcinoma / immunology*,  metabolism,  pathology*
Interleukin-17 / physiology*
Neoplasm Invasiveness*
RNA, Messenger / biosynthesis
Receptors, Interleukin / biosynthesis,  genetics,  physiology
Receptors, Interleukin-17
Trophoblasts / immunology,  metabolism,  pathology*
Reg. No./Substance:
0/IL17RA protein, human; 0/Interleukin-17; 0/RNA, Messenger; 0/Receptors, Interleukin; 0/Receptors, Interleukin-17

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