Document Detail


The effect of insulin on the intracellular distribution of 14(R,S)-[18F]Fluoro-6-thia-heptadecanoic acid in rats.
MedLine Citation:
PMID:  16791750     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The aim of this study was to determine the effect of hyperinsulinemia on myocardial and hepatic distribution and metabolism of 14(R,S)-[18F]fluoro-6-thia-heptadecanoic acid ([18F]FTHA). PROCEDURES: Mitochondrial retention and intracellular lipid incorporation of [18F]FTHA were compared to that of [14C]-2-bromopalmitate or [14C]palmitate during hyperinsulinemic clamp vs. saline infusion in male Wistar rats. RESULTS: Mitochondrial 18F activity was increased in the heart (1.7 +/- 0.4 vs. 0.5 +/- 0.1% ID/g, P < 0.05), whereas it was reduced in the liver (1.1 +/- 0.3 vs. 1.8 +/- 0.4% ID/g, P < 0.05) during insulin vs. saline infusion, respectively. Mitochondrial [14C]-2-bromopalmitate activity was affected by insulin in a similar way in both tissues. The fractional esterification of [18F]FTHA into triglycerides was impaired compared to [14C]palmitate in both tissues, and [18F]FTHA was insensitive to the shift of esterification of fatty acids into complex lipids in response to insulin. CONCLUSIONS: [18F]FTHA is sensitive to insulin-induced modifications of free fatty acid oxidative metabolism in rats but is insensitive to changes in nonoxidative fatty acid metabolism.
Authors:
Xiuli Ci; Frédérique Frisch; François Lavoie; Pascale Germain; Roger Lecomte; Johan E van Lier; François Bénard; André C Carpentier
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  8     ISSN:  1536-1632     ISO Abbreviation:  Mol Imaging Biol     Publication Date:    2006 Jul-Aug
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Endocrinology, Centre hospitalier universitaire de Sherbrooke, Université de Sherbrooke, Sherbrooke, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Availability
Blood Glucose / analysis
Fatty Acids / pharmacokinetics*
Hyperinsulinism / metabolism
Insulin / blood,  pharmacology*
Lipid Metabolism / drug effects
Liver / metabolism*
Male
Mitochondria, Heart / metabolism
Mitochondria, Liver / metabolism
Myocardium / metabolism*
Palmitates / diagnostic use
Rats
Rats, Wistar
Tissue Distribution
Whole-Body Irradiation
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids; 0/Palmitates; 11061-68-0/Insulin; 138225-06-6/14-fluoro-6-thiaheptadecanoic acid; 18263-25-7/2-bromopalmitate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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