Document Detail

The effect of inositol 1,3,4,5-tetrakisphosphate on inositol trisphosphate-induced Ca2+ mobilization in freshly isolated and cultured mouse lacrimal acinar cells.
MedLine Citation:
PMID:  10727404     Owner:  NLM     Status:  MEDLINE    
Earlier reports have shown a remarkable synergism between InsP(4) and InsP(3) [either Ins(1,4,5)P(3) or Ins(2,4,5)P(3)] in activating Ca(2+)-dependent K(+) and Cl(-) currents in mouse lacrimal cells [Changya, Gallacher, Irvine, Potter and Petersen (1989) J. Membr. Biol. 109, 85-93; Smith (1992) Biochem. J. 283, 27-30]. However, Bird, Rossier, Hughes, Shears, Armstrong and Putney [(1991) Nature (London) 352, 162-165] reported that they could see no such synergism in the same cell type. A major experimental difference between the two laboratories lies in whether or not the cells were maintained in primary culture before use. Here we have compared directly the responses to inositol polyphosphates in freshly isolated cells versus cells cultured for 6-72 h. In the cultured cells, Ins(2,4,5)P(3) at 100 microM produced a robust stimulation of K(+) and Cl(-) currents, as much as an order of magnitude greater than that observed in the freshly isolated cells. However, the freshly isolated cells could be restored to a sensitivity similar to cultured cells by the addition of InsP(4) at a concentration two orders of magnitude lower than that of Ins(2,4,5)P(3). We discuss the implications of this with respect to the actions of InsP(4), including the possibility that disruption of the cellular structure during the isolation of the cells exposes an extreme manifestation of a possible physiological role for InsP(4) in controlling calcium-store integrity.
P M Smith; A R Harmer; A J Letcher; R F Irvine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  347 Pt 1     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-06-05     Completed Date:  2000-06-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Department of Clinical Dental Sciences, University of Liverpool, Liverpool L69 3BX, U.K.
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MeSH Terms
Acetylcholine / pharmacology
Calcium / metabolism*
Cells, Cultured
Chloride Channels / drug effects,  physiology
Inositol 1,4,5-Trisphosphate / pharmacology*
Inositol Phosphates / pharmacology*
Lacrimal Apparatus / cytology*,  drug effects,  physiology*
Potassium Channels / drug effects,  physiology
Time Factors
Reg. No./Substance:
0/Chloride Channels; 0/Inositol Phosphates; 0/Potassium Channels; 102850-29-3/inositol-1,3,4,5-tetrakisphosphate; 51-84-3/Acetylcholine; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; 91840-07-2/inositol 2,4,5-trisphosphate

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