| The effect of induced sputum and bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease on neutrophil migration in vitro. | |
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MedLine Citation:
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PMID: 20679746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of study was to investigate a chemotactic effect of induced sputum and bronchoalveolar lavage fluid on blood neutrophils in patients with chronic obstructive pulmonary disease (COPD) and healthy individuals. MATERIAL AND METHODS: Forty-three smokers with COPD, 19 ex-smokers with COPD, 13 healthy smokers, and 17 healthy nonsmokers were recruited to the study. Neutrophils were isolated from peripheral blood of study individuals. For the same experimental conditions, pooled induced sputum and bronchoalveolar lavage fluid of 20 COPD patients were used. Neutrophil chemotaxis in vitro was performed in cell-transmigration chamber. Substances tested for chemoattraction (interleukin-8, induced sputum, bronchoalveolar lavage fluid directly or in addition to interleukin-8) were added to lower wells. Upper wells were filled with 2.5 x 10(6)/mL of neutrophil culture and incubated for 2 hours. Migration was analyzed by flow cytometry. RESULTS: Interleukin-8 (10-100 ng/mL) induced a dose-dependant neutrophil migration in all the groups. Only 100 ng/L of interleukin-8 induced more intensive chemotaxis of neutrophils from COPD smokers as compared to ex-smokers (P<0.05). Such difference between healthy individuals was obtained using 30 ng/mL of interleukin-8 (P<0.05). Induced sputum/interleukin-8 (10-100 ng/mL), as well as induced sputum directly, induced neutrophil migration (P<0.05). Chemotaxis of neutrophils isolated from COPD patients and healthy nonsmokers did not depend on additional interleukin-8 concentration. Bronchoalveolar lavage fluid/interleukin-8 (30-100 ng/mL) induced more intensive migration of neutrophils from COPD patients than bronchoalveolar lavage fluid (P<0.05) alone. CONCLUSIONS: Migration of neutrophils isolated from patients with COPD was more intensive compared to healthy individuals. Induced sputum and bronchoalveolar lavage fluid directly and with addition of interleukin-8 stimulated chemotaxis, and it was higher in neutrophils from COPD patients. Migration of neutrophils did not depend on smoking status. |
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Authors:
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Agne Babusyte; Jolanta Jeroch; Rimantas Stakauskas; Kristina Stravinskaite; Kestutis Malakauskas; Raimundas Sakalauskas |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article |
Journal Detail:
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Title: Medicina (Kaunas, Lithuania) Volume: 46 ISSN: 1648-9144 ISO Abbreviation: Medicina (Kaunas) Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-10-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9425208 Medline TA: Medicina (Kaunas) Country: Lithuania |
Other Details:
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Languages: eng Pagination: 315-22 Citation Subset: IM |
Affiliation:
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Laboratory of Pulmonology, Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 4, 50161 Kaunas, Lithuania. agne.babusyte@gmail.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Bronchoalveolar Lavage Fluid Data Interpretation, Statistical Female Forced Expiratory Volume Humans Interleukin-8 / blood Male Middle Aged Neutrophil Activation Patient Selection Pulmonary Disease, Chronic Obstructive* / diagnosis, physiopathology Smoking* / adverse effects Smoking Cessation Spirometry Sputum Statistics, Nonparametric Vital Capacity |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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