| The effect of imatinib mesylate on the proliferation, invasive ability, and radiosensitivity of retinoblastoma cell lines. | |
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MedLine Citation:
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PMID: 23154488 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PurposeOur aim was to evaluate the potential effect of imatinib mesylate (IM), a small molecule that specifically inhibits the tyrosine quinase receptors, on the proliferation and invasive abilities of two human retinoblastoma (Rb) cell lines. Furthermore, the ability of IM to radiosensitize Rb cells was evaluated. The potential targets of IM (C-kit, PDGRF-α and -β, and c-Abl) were also investigated in these cell lines.MethodsTwo human Rb cell lines (WERI-RB-1 and Y79) were cultured under normal growth conditions. An MTT-based proliferation assay and a Matrigel invasion assay were performed with and without exposure to 10 μM of IM. The cells were also irradiated with graded dosages of 0, 2, 4, 6, 8, and 10 Gy with and without IM and their proliferations rates were analyzed. Western blot and immunocytochemical analysis of cytospins were performed to evaluate the expression of C-kit, PDGRF-α and -β, and c-Abl.ResultsWhen IM was added to both cell lines a statistically significant (P<0.05) reduction in proliferation and invasive ability were observed. Exposure to IM also significantly increased the radiosensitivity of both Rb cell lines. The c-Abl expression was strongly positive, PDGRF-α and -β expression were also positive but the C-kit expression was negative in both cell lines.ConclusionsThese results indicate that Gleevec may be useful as an adjuvant treatment in Rb patients, specially those considered for radiation therapy.Eye advance online publication, 16 November 2012; doi:10.1038/eye.2012.231. |
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Authors:
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L R de Moura; J-C Marshall; S Di Cesare; B F Fernandes; E Antecka; M N Burnier |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-16 |
Journal Detail:
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Title: Eye (London, England) Volume: - ISSN: 1476-5454 ISO Abbreviation: Eye (Lond) Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703986 Medline TA: Eye (Lond) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Department of Ophthalmology and Pathology, The McGill University Health Center and Henry C Witelson Ocular Pathology Laboratory, Montreal, Quebec, Canada [2] Instituto Brasileiro de Oftalmologia, Rio de Janeiro, Brazil [3] Department of Ophthalmology, Federal University of São Paulo-UNIFESP/EPM, São Paulo, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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