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The effect of hyperglycemia on neonatal immune responses in-vitro.
MedLine Citation:
PMID:  21366510     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Introduction.?Acute hyperglycemia is considered as a pro-inflammatory state and is related to an adverse outcome in critically ill adults. Neonates are susceptible to infections and systemic inflammatory response syndrome induced by pro-inflammatory cytokines. This study focuses on the interaction between neonatal glucose homeostasis and the pro-inflammatory cytokine production in term and preterm infants in-vitro. Methods.?We analyzed the pro-inflammatory cytokine production in whole cord blood of term infants (n?=?10), preterm infants > 32 weeks (n=16) and preterm infants??32 weeks of gestational age (n?=?13) and in adult controls (n=14) using an in-vitro sepsis-model. Whole blood was pre-incubated with different concentrations of glucose (0?1000 mg/dl) and insulin (0?62.5 IE/l) and stimulated with lipopolysaccharide. The intracytoplasmatic TNF-?, IL-6, and IL-8 response was measured by flow cytometry. Results.?In-vitro hyperglycemia induced a dose-dependent increase of IL-8 in all age groups while TNF-? was demonstrated to be stimulated by glucose in cord blood samples of preterm infants??32 weeks of gestational age and term infants. In contrast, insulin showed no significant effects on pro-inflammatory cytokine production in-vitro. Conclusion.?Acute hyperglycemia may induce pro-inflammatory cytokine responses in neonatal whole blood in-vitro. These data provide a basis for further in-vitro signal transduction studies and in-vivo investigations about the significance of neonatal glucose homeostasis and its impact on long-term outcome of this susceptible patient cohort.
Authors:
Petra Temming; Birte Tr Ger; Susanne Thonnissen; Paul-Martin Holterhus; Christian Schultz; Christoph H Rtel
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-3
Journal Detail:
Title:  The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians     Volume:  -     ISSN:  1476-4954     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101136916     Medline TA:  J Matern Fetal Neonatal Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Paediatrics, University Hospital Schleswig-Holstein, Campus L?beck, Germany.
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