| The effect of hydrolysis time on amino acid analysis. | |
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MedLine Citation:
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PMID: 16001867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Determining the amino acid content of a protein involves the hydrolysis of that protein, usually in acid, until the protein-bound amino acids are released and made available for detection. Both the variability in the ease of peptide bond cleavage and differences in the acid stability of certain amino acids can significantly affect determination of a protein's amino acid content. By using multiple hydrolysis intervals, a greater degree of accuracy can be obtained in amino acid analysis. Correction factors derived by linear extrapolation of serial hydrolysis data are currently used. Compartmental modeling of the simultaneous hydrolysis (yield) and degradation (decay) of amino acids by nonlinear multiple regression of serial hydrolysis data has also been validated and applied to determine the amino acid composition of various biological samples, including egg-white lysozyme, human milk protein, and hair. Implicit in the routine application of serial hydrolysis in amino acid analysis, however, is an understanding that correction factors, derived either linearly or through the more accurate nonlinear multiple regression approach, need to be determined for individual proteins rather than be applied uniformly across all protein types. |
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Authors:
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Alison J Darragh; Paul J Moughan |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Journal of AOAC International Volume: 88 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2005 May-Jun |
Date Detail:
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Created Date: 2005-07-08 Completed Date: 2005-09-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 888-93 Citation Subset: IM |
Affiliation:
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Massey University, Institute of Food, Nutrition, and Human Health, Private Bag 11 222, Palmerston North, New Zealand. a.darragh@massey.ac.nz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Animals Chemistry Techniques, Analytical / methods* Chickens Egg White Hair / metabolism Humans Hydrolysis Milk Proteins / analysis Milk, Human / metabolism Models, Statistical Muramidase / chemistry Regression Analysis Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Milk Proteins; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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