Document Detail

The effect of human eosinophil granule major basic protein on airway responsiveness in the rat in vivo. A comparison with polycations.
MedLine Citation:
PMID:  8466137     Owner:  NLM     Status:  MEDLINE    
Major basic protein (MBP) is a highly cationic protein found in the granules of eosinophils. It has been postulated that MBP may participate in the pathogenesis of airway hyperresponsiveness exhibited by asthmatic patients. Accordingly, we used a rat model to investigate the effect of human MBP instillation on airway responsiveness and the possible role of cationic charge in the determination of this effect. Dose-response characteristics to inhaled methacholine (MDRC) were determined at baseline, and the animals were allowed to recover. Then animals in the experimental group received 100 micrograms of purified human MBP via direct instillation into the trachea. One hour after instillation, the MDRC were again assessed. Control animals received (in lieu of MBP) buffer from the void volume pool of the same chromatography column used to purify the MBP. One hour after instillation of MBP there was a significant increase in airway responsiveness to inhaled methacholine, whereas control animals exhibited no increase in airway responsiveness. Some animals from the MBP group were restudied 48 h after MBP instillation, by which time airway responsiveness had returned to baseline level. The effect of the polycations poly-L-arginine and poly-L-lysine on airway responsiveness was also examined. As with MBP, airway responsiveness to inhaled methacholine increased 1 h after the instillation of either polycation. In addition, acetylation of the charged groups on poly-L-lysine resulted in a loss of this effect. Histologic examination of the airways failed to reveal airway epithelial shedding 1 h after MBP or polycation instillation.(ABSTRACT TRUNCATED AT 250 WORDS)
D A Uchida; S J Ackerman; A J Coyle; G L Larsen; P F Weller; J Freed; C G Irvin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  147     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-04-30     Completed Date:  1993-04-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  982-8     Citation Subset:  AIM; IM    
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado.
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MeSH Terms
Airway Resistance / drug effects
Blood Proteins / pharmacology*
Bronchial Hyperreactivity / physiopathology*
Bronchial Provocation Tests
Dose-Response Relationship, Drug
Eosinophil Granule Proteins
Lung / drug effects,  pathology
Methacholine Chloride / pharmacology
Peptides / pharmacology
Polylysine / analogs & derivatives,  pharmacology
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Blood Proteins; 0/Cations; 0/Eosinophil Granule Proteins; 0/Peptides; 152473-69-3/N-acetyl polylysine; 25104-18-1/Polylysine; 25212-18-4/polyarginine; 62-51-1/Methacholine Chloride; EC 3.1.-/Ribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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