Document Detail


The effect of human GATA4 gene mutations on the activity of target gonadal promoters.
MedLine Citation:
PMID:  19008335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GATA transcription factors are crucial regulators of cell-specific gene expression in many tissues including the gonads. Although clinical cases of reproductive dysfunction have yet to be formally linked to GATA gene mutations, they have begun to be reported in other systems. Heterozygous GATA4 mutations have been associated with cases of congenital heart defects. Little is known, however, about the effect of these mutations on gonadal gene transcription. Since individuals carrying these mutations do not appear to suffer from gross reproductive defects, we hypothesized that this might be due to the differential transcriptional properties of the mutant proteins on heart versus gonadal target genes. Five mutations (S52F, E215D, G295S, V266M, and E359X) were recreated in the rat GATA4 protein. Several parameters were used to analyze the transcriptional properties of the mutants: activation of known gonadal target promoters (Star, Cyp19a1, and Inha), DNA binding, and interaction with GATA4 transcriptional partners. Three mutations (S52F, G295S, and E359X) reduced GATA4 transcriptional activity on the different gonadal promoters. With the exception of the G295S mutant, which showed a significant loss of DNA-binding affinity, the decrease in activity of the other GATA4 mutants was not associated with a change in DNA binding. All GATA4 mutants retained their ability to interact and cooperate with their major gonadal partners (NR5A1 and NR5A2) thereby compensating in part for the loss in intrinsic GATA4 transcriptional activity. Thus, unlike the heart, where the GATA4 mutations have deleterious effects, our data suggest that they would have a lesser impact on gonadal gene transcription and function.
Authors:
Marie France Bouchard; Hiroaki Taniguchi; Robert S Viger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-13
Journal Detail:
Title:  Journal of molecular endocrinology     Volume:  42     ISSN:  1479-6813     ISO Abbreviation:  J. Mol. Endocrinol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-29     Completed Date:  2009-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8902617     Medline TA:  J Mol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  149-60     Citation Subset:  IM    
Affiliation:
Reproduction, Perinatal and Child Health, Centre de Recherche du Centre Hospitalier Universitaire de Québec, 2705 Laurier Boulevard, Québec City, Québec, Canada G1V 4G2.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Line
Cyclic AMP-Dependent Protein Kinases / metabolism
DNA / metabolism
GATA4 Transcription Factor / chemistry,  genetics*
Gonads / enzymology,  metabolism*
Humans
Male
Mice
Molecular Sequence Data
Mutant Proteins / metabolism
Point Mutation / genetics*
Promoter Regions, Genetic*
Protein Binding
Rats
Receptors, Cytoplasmic and Nuclear / metabolism
Steroidogenic Factor 1 / metabolism
Transcription, Genetic
Transcriptional Activation
Chemical
Reg. No./Substance:
0/GATA4 Transcription Factor; 0/GATA4 protein, human; 0/Mutant Proteins; 0/Nr5a2 protein, mouse; 0/Receptors, Cytoplasmic and Nuclear; 0/Steroidogenic Factor 1; 0/steroidogenic factor 1, mouse; 9007-49-2/DNA; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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